Fig 1.
Position of each primer used for PCR amplification.
Table 1.
Primers used in this study.
Fig 2.
Phase-contrast micrographs of Sf9 cells transfected or infected with bApGOZA or AcMNPV, respectively, at 7 days.
Cells were transfected with bApGOZA DNA or infected with AcMNPV at an MOI of 1 PFU/cell. Bar markers represent 20 ㎛.
Fig 3.
PCR analysis for the identification of viral contamination.
PCR amplifications were performed for AcMNPV, vApGOZA, bApGOZA and pUC19 DNA using each primer shown in Fig 1 and Table 1.
Fig 4.
PCR and RT-PCR analyses for ORF1629.
PCR amplifications were performed for AcMNPV, vApGOZA and bApGOZA DNA using each primer shown Fig 1 and Table 1 (A, B). Sf9 cells were infected with AcMNPV or vApGOZA at an MOI of 5 PFU/cell. Total RNA from infected cells was collected and subjected to RT-PCR, and the products were analyzed by electrophoresis on 1% agarose gels. PCR amplification was performed on DNase-treated total RNA (C). RT-PCR was performed on the cDNA synthesized using truncated (D) and complete (E) ORF1629 gene-specific primers.
Fig 5.
Comparison of viral growth between wild-type and recombinant viruses.
Sf9 (A) and Bm5 (B) cells were infected with each virus at an MOI of 1 PFU/cell. Cell culture supernatants were harvested and titrated using TCID50 end-point dilution assays. The bars indicate the mean ± SE (n = 3).
Fig 6.
Comparison of OB yields for each virus.
Sf9 (A) and Bm5 (B) cells infected with each virus at an MOI of 1 PFU/cell. Yield of total produced OBs was determined using a hemocytometer at 3, 4 and 5 days p. i.