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Fig 1.

Sequence diagram of MEPI-SAGE.

Several slices are exited simultaneously using MB RF. An additional gradient blip scheme in slice direction with each phase encoding step shifts the slices in the PE direction and reduces the high g-factor penalties and effectively reduces the noise amplification in the reconstructed images. The rephasing gradient (in red) was inserted after GE readout to return the data acquisition to the k-space center.

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Table 1.

Patient characteristics.

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Table 2.

P-values for comparisons of VAI parameter distributions between HGG tumor core and peritumor, edema, and cNAB.

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Table 3.

Correlation coefficients and p-values between pairs of VAI parameter distributions in the tumor core (N = 15).

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Table 4.

Correlation coefficients and p-values between pairs of VAI parameter distributions in the normal tissue (N = 15).

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Fig 2.

GE (a) /SE (b) EPI-based images acquired with single and multiband excitations. All images were reconstructed with an unfolding procedure for three-fold in-plane parallel imaging. With use of the multiband technique, the number of slices was increased to 24 (versus 12 slices in the singleband data). Using a multiband excitation with a multiband factor of 2 as well as blipped-CAIPI, two simultaneously excited slices were folded together with a phase shift. After reconstruction with the 'Slice-GRAPPA' algorithm [19], these two-folded slices can be clearly separated. Subtraction of multiband EPI from singleband EPI produces only little differences at the same slice location.

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Fig 3.

G-factor maps of GE (a) and SE (b), induced from the MB acceleration. The g-factor noise penalty, induced from the MB acceleration of all subjects, has an averaged value of 1.35 and 1.33 for GE and SE imaging, respectively. This relative low g-factor was achieved using a blipped-CAIPI technique.

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Fig 4.

Histograms of equally located image slabs for GE readout intensity using multiband (MB) (a) and singleband (SB) (b) techniques, and for SE readout intensity using MB (c) and SB (d) techniques. The averaged correlation coefficient for MB and SB histograms across all patients was found as 0.96 ± 0.03 for GE, and 0.96 ± 0.04 for SE readout intensity, respectively (N = 32).

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Fig 5.

(a) Relaxation rate time curves from the voxel in the region of the tumor core. The GE signal peaks earlier than the SE signal, resulting in a clockwise loop when plotting the relaxation rate curves in a point-by-point time-parametrized plot (b). (c) The slope length map visualizes the long axis of the loop. (XE = GE, SE; see also Eq (1) in the methods section).

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Fig 6.

(a) Contrast-enhanced T1-weighted MPRAGE image. (b) FLAIR image. (c) rCBV. (d) Slope length. (e) Short axis. (f) Slope. (g) Distance map. (h) MTI. (i) VIPS. (j) Vessel size. (k) Q map.

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Fig 7.

Box-whisker plots (whiskers: Minimum, maximum; box: 25th to75th percentile; line: Mean value) for VAI parameters I, MTI, VIPS, slope length, slope; short axis, rCBV in tumor core; peritumor (non-edematous); edema; cNAB.

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Fig 8.

Linear correlation between VAI parameters I and MTI in the tumor core of HGG (red circle).

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