Fig 1.
Map of the Bocas del Toro Archipelago.
The Black box indicates the sampling site that corresponds to Hospital Point in Solarte Island. Modified from Seemann et al. (2014).
Fig 2.
Map of localities and sampling replication.
Bocas del Toro (BDT COI and CR). COI sequences: Alabama (AL), Bagdad Mexico- Gulf of Mexico (BM), Belize (BZ), Florida (FL), South Carolina (SC), Texas (TX). CR sequences: Florida Bay, Florida (FB), Tampa Bay, Florida (TB), Panama City, Florida (PC), and Belize (BZ).
Fig 3.
Maximum parsimony network of the cytochrome oxidase I (COI) for S. tiburo.
Circles are sized proportional to haplotype frequency and color coded for location. Sample sizes are as follows: Bocas del Toro (BDT = 15) and GenBank sequences: Alabama (AL = 1), Bagdad Mexico (BM = 4), Belize (BZ = 16), Florida (FL = 16), South Carolina (SC = 4), Texas Gulf of Mexico (TX = 4).
Fig 4.
Maximum likelihood distance tree of COI haplotype sequences from S. tiburo and outgroups: S. lewini, S. tudes and C. leucas.
Clade A corresponds to BDT and BZ. Clade B corresponds to the localities of the WA and the Gulf of Mexico.
Table 1.
Eight variable sites over 639 bp of the mitochondrial COI gene determining 3 haplotypes for S. tiburo.
Table 2.
Pairwise FST values (above diagonal) and ΦST values (below diagonal) for the COI gene from the Atlantic and Caribbean populations of S. tiburo.
Bocas del Toro (BDT), and other localities from GeneBank: Bagdad Mexico (BM), Belize (BZ), Florida (FL), South Carolina (SC) and Texas (TX).
Fig 5.
Maximum parsimony network for S. tiburo CR haplotypes.
Circles are sized proportional to haplotype frequency and color coded for location. Small black dots and hatch marks along the branches represent mutational steps that were not observed in this study. Sample sizes are as follows: Bocas del Toro (BDT = 15), Belize (BZ = 54), and GenBank sequences: North Carolina (NC = 23), Florida Bay (FB = 25), Tampa Bay (TB = 27), Panama City (PC = 25). For details and GenBank accession numbers see S2 Table.
Fig 6.
Maximum likelihood distance tree for CR haplotype sequences from S. tiburo, and outgroups: S. media (Eastern Caribbean Panama—GU385317.1), and S. lewini (WA—KY315830.1).
Bocas del Toro (BDT = H45-54) and most common haplotypes from the WA from GenBank sequences from North Carolina (NC), Tampa Bay (TB), Florida Bay (FB) and Panama City (PC). ML support values are given in the branches.
Table 3.
Twelve variable sites over 1064 bp of the mitochondrial CR gene determining 10 haplotypes for S. tiburo.
Table 4.
Pairwise FST values (above diagonal) and ΦST values (below diagonal) for the CR, of S. tiburo from Bocas del Toro (BDT), Belize (BZ) and GeneBank sequences from the WA: North Carolina (NC), Florida bay (FB), Tampa Bay (TB), Panama City (PC).
Table 5.
AMOVA analysis using pairwise genetic distances and conventional FST estimates.