Fig 1.
Map of sampling sites on the South Fork Eel River and on the Russian River in California, USA.
Blue areas on the map refer to the Eel River watershed, while green denotes the Russian River watershed. Sites are labelled in order of increasing drainage area from 1 to 5, while the suffix refers to the river: ELD = Elder Creek, SFE = South Fork Eel, RUS = Russian River.
Table 1.
Physicochemical parameters collected at each sampling site.
Fig 2.
Anatoxin and microcystin/nodularin concentrations vary by site.
(A) Summed anatoxin (the total of the four congeners; anatoxin-a, homoanatoxin-a, dihydroanatoxin-a and dihydrohomoanatoxin-a); and (B) summed microcystins and nodularin-R from 10 replicate attached periphyton samples (n = 5 for Cladophora-dominated samples; 3_SFE_C). collected from each of four sites on the Eel River on 29 July 2018 and 9 attached periphyton samples from one site on the Russian River on 31 July 2018. Colours represent the dominant taxa in mats collected from each site. dw = dry weight. Note log scale on the y-axes. Lines within the boxes are medians, the ends of boxes are quartiles and whiskers extend to the lowest or highest data point ≤ 1.5 × the interquartile range. Black dots are outliers. A Kruskal-Wallis test and pairwise Wilcoxon rank sum test with a Benjamini-Hochberg adjustment was used to identify sites that were significantly different from one another (p <0.05), denoted by the letter above the plot. Dotted lines represent the analytical limit of quantitation and bold dashed lines the analytical limit of detection.
Fig 3.
The composition of anatoxin congeners from attached periphyton samples collected at the five sites.
Each vertical bar represents one periphyton sample and the vertical black lines delineate each site. 3_SFE_C represents Cladophora-dominated samples at site 3_SFE. ATX = anatoxin-a, HTX = homoanatoxin-a, dhATX = dihydroanatoxin-a, dhHTX = dihydrohomoanatoxin-a.
Fig 4.
Anatoxin concentrations, anaC gene copies and anatoxin quota among three sites on the Eel and Russian rivers.
(A) Summed anatoxin concentrations; (B) anaC gene copies; and (C) anatoxin quota, from five attached periphyton samples collected from two sites on the Eel River and one site on the Russian River. dw = dried weight. Note the different y-axis scales. See Fig 2 for interpretation of boxplots. A Kruskal-Wallis test and pairwise Wilcoxon rank sum test with a Benjamini-Hochberg adjustment was used to identify sites that were significantly different from one another (p <0.05), denoted by the letter above the plot.
Fig 5.
Anatoxin concentrations, anaC gene copies and anatoxin quota among fine-scale samples from three rocks at 4_SFE on the Eel river.
(A) Summed anatoxin concentrations; (B) anaC gene copy concentrations; and (C) anatoxin quota from five mat samples collected from each of three rocks at 4_SFE. dw = dried weight. Note the different y-axis scales. See Fig 2 for interpretation of boxplots. A Kruskal-Wallis test and pairwise Wilcoxon rank sum test with a Benjamini-Hochberg adjustment was used to identify rocks that were significantly different from one another (p <0.05), denoted by the letter above the plot.