Fig 1.
In vivo quantification of tumor growth by bioluminescence tracking of luciferase expressing CT-26 cells over 15 days.
(A) Microphotographs of anesthetized mice for each group inside the bioluminescent imaging box. (B) Tumor growth evaluation based on bioluminescence measurements for the four groups (IV, SC, IP, A) at day 3, day 9, day 12 and day 15 (low values are not always visible on the graph). (C) PCI score at day 15 post-grafting. In summary, the mice having undergone laparotomy surgery (group A: intraperitoneal cell injection after peritoneum aggression) present an extensive tumoral growth with the highest peritoneal carcinomatosis index (PCI). Furthermore, statistically significant differences are observed between the four groups. n = 5 for each group, mean +- SD. * for p value <0.05, ** <0.01, *** <0.001 and ****<0.0001.
Fig 2.
Images after mouse laparotomy at day 15 post-grafting, (A) A group showed extensive intraperitoneal tumor growth localized majority on the parietal peritoneum and the diaphragm, and (B) IP group displayed a limited peritoneal metastasis localized on the visceral peritoneum. (C) HES staining in the intestinal bowel with peritoneal tumoral nodule.
Fig 3.
(A-B) HES staining and (C-G) immunohistochemistry for CD3, CD4, CD8 of a 15 days grafted CT-26+ nodule in a mouse from IP group. Grafted nodules reveal high number of cells including Tumor infiltrating Lymphocytes (TILs) among a complex tumoral microenvironment (high vascularization, poor fibrosis, dense tissue). Multiphoton imaging of tumoral tissue (H) and normal peritoneum (I-L). v = vessel, LT = T Lymphocyte, LT4 = T4 Lymphocyte, LTc = cytotoxic Lymphocyte, lu = lumen, SHG = second harmonic generation. Scale bar in a applied to c,e,f,g, in b applied to d, and in h applied to i-l.