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Table 1.

Hypothetical epidemiological scenarios for evaluating RITA performance in HIV incidence surveillance.

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Table 2.

Calibrator reactivity and reproducibility of results assessed by repeat testing.

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Fig 1.

Maxim vs. Sedia OD and ODn measurements.

A: Maxim vs. Sedia Optical Density (OD); B: Maxim vs. Sedia normalized Optical Density (ODn). The blue lines are linear regression fits and the red dashed lines show the diagonal (slope if the two assays produced equivalent results). C: Bland-Altman plot for Optical Density (OD); D: Bland-Altman plot for normalized Optical Density (ODn). The red lines represent zero bias, the blue solid lines the mean differences and the blue dashed lines the 95% lower and upper limits.

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Table 3.

MDRI estimates for Maxim and Sedia LAg assays by HIV-1 subtype and ODn threshold, using supplemental viral load threshold of >1,000c/mL.

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Table 3 Expand

Fig 2.

Context-specific false-recent rate (FRR) against MDRI in three demonstrative surveillance scenarios.

A: Scenario similar to South African epidemic. B: Scenario similar to Kenyan epidemic. C: Concentrated epidemic scenario. A supplementary viral load threshold of >1,000c/mL is used throughout. We assume ARV exposure testing classifies all treated individuals as long-term. This assumption is relaxed in S4 Fig.

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Fig 3.

Relative standard error (RSE) of incidence estimate against ODn threshold in three demonstrative surveillance scenarios.

A: Scenario similar to South African epidemic. B: Scenario similar to Kenyan epidemic. C. Concentrated epidemic scenario. A supplementary viral load threshold of >1,000c/mL is used throughout. We assume ARV exposure testing classifies all treated individuals as long-term. This assumption is relaxed in S5 Fig.

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Fig 3 Expand

Table 4.

Summary recommendations for use of the Maxim and Sedia LAg assays.

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Table 4 Expand