Table 1.
Characteristics of the polymerase chain reaction assays.
Fig 1.
Agarose gel showing PCR amplification products from male DNA serial dilutions.
Two-fold serial dilutions of male DNA were amplified by the means of (A) s-SRY, (B) s-TSPY, and (C) n-TSPY. Lane 1, no dilution; lanes 2 to 13 show one-in-two of the two-fold male DNA dilutions (from 1:2 to 1:838,9 x 104); lane 14, female DNA; lane 15, no template control; lane L, 100–1500 bp DNA ladder (Bio Basic, Markham, Ontario, CA).
Table 2.
Socio-demographic characteristics and behaviours at baseline (n = 45).
Table 3.
Prevalence of recent semen exposure according to the compared assays.
Fig 2.
Agarose gel showing standard and nested PCR products size.
Female (-) and male (+) DNA extracted from peripheral blood mononuclear cells were amplified with a standard PCR targeting SRY (s-SRY), a standard PCR targeting TSPY (s-TSPY), and a nested PCR targeting TSPY (n-TSPY), respectively. Lane L, 100–1500 bp DNA ladder (Bio Basic, Markham, Ontario, CA).
Table 4.
Performance of four assays of semen exposure compared to the nested TSPY PCR assay (n = 43).