Table 1.
Xanthomonas strains used in this study.
Table 2.
Genome sequence information and statistics of X. arboricola pv. pruni strains CITA 9 and CITA 99.
Fig 1.
Genome comparative analysis between Xanthomonas arboricola pv. pruni (Xap) and X. citri subsp. citri (Xcc).
(A) Distribution of unique gene clusters per genome sequence. (B) Gene clusters shared and unique to each Xanthomonas species. (C) Cluster comparative analysis (bootstrap values showed at the branch points) and (D) principal component analysis spanned by the two principal components based on the presence/absence of 7,506 gene clusters in the 50 genome sequences of Xap and Xcc.
Fig 2.
Relative frequency distribution of the unique gene clusters found in the genome sequences of seven strains of X. arboricola pv. pruni (Xap) and 43 strains of X. citri subsp. citri (Xcc) as well as those gene clusters shared by both bacterial species (Xcc + Xap).
Only those gene clusters that were complete according to the annotation obtained from the COG database were counted and represented (81, 92 and 2,402 protein sequences for Xap, Xcc and Xcc+Xap respectively).
Fig 3.
Dendrogram based on the profiles for methyl accepting chemotaxis proteins (MCPs) (A), sensors of the two-component regulatory system (STCRs) (B) and TonB- dependent transporters (TBDT) (C) in Xanthomonas citri subsp. citri (Xcc) and Xanthomonas arboricola pv. pruni (Xap). Similarities were calculated, based on sensor presence/absence data converted to a binary form, according to the Jaccard´s coefficient and clustering achieved by UPGMA using the NTSYS version 2.1.
Fig 4.
Distribution of the type III effectors (T3Es) and other type III secreted proteins (T3SPs) among the bacterial strains of X. arboricola pv. pruni (Xap, 7 strains) and X. citri subsp. citri (Xcc, 43 strains).
Color scale indicates the percentage of strains containing each one of the searched proteins.
Fig 5.
Biofilm formation by Xanthomonas citri subsp. citri strain 306 and Xanthomonas arboricola pv. pruni strain CITA 33 on polypropylene surface quantified by absorbance of crystal violet stain.
Data were analyzed as shown in the main text.
Fig 6.
Scanning electron microscopy of Xanthomonas citri subsp. citri strain 306 and Xanthomonas arboricola pv. pruni strain CITA 33 onto the leaf surface of Citrus lemon (CL) and Prunus dulcis (PD) after 4 and 7 dpi.
Scale bar 5 μm.
Fig 7.
Survival of Xanthomonas citri subsp. citri strain 306 and Xanthomonas arboricola pv. pruni strain CITA 33 onto the leaf surface of C. lemon (CL) and P. dulcis (PD) quantified by isolation and serial dilutions at 4 and 7 dpi.
Mean from three replicates with the standard deviation are represented in each bar.