Table 1.
Relative amount of Sdc1 compared to total protein for gender, age and relationship in Sdc1-/-, Sdc1+/- and WT mice.
Table 2.
Total number of animals used for the blood analysis assays.
Fig 1.
A representative Swiss-roll of the ileum of a WT female mouse.
(A) The outer end of the Swiss-roll is the beginning of the intestinal part. A villus (B), a crypt (C) and a musculature site (D) are shown (length is indicated as bar).
Fig 2.
Amount of Sdc1 protein in tail biopsies of Sdc1-/-, Sdc1+/- and WT mice measured by ELISA and further normalized to the total protein (***p<0.01).
Fig 3.
Weight assessment of adult Sdc1+/- and WT males and females born either after normal mating or vice versa ET (***p<0.01).
Fig 4.
Metabolic parameters of male and female Sdc1+/- and WT mice.
Corticosterone (A, B), glucose (C, D), insulin (E, F) and leptin (G, H) were measured at 6 weeks and 6 months of age (*p<0.05; ***p<0.01;).
Fig 5.
Manually measured food and water consumption of adult Sdc1+/- and WT animals per week.
(A, B) Average food consumption in g/g BW. (C, D) Average water intake in ml/g BW (*p<0.05).
Fig 6.
Comparison of manually and PhenoMaster automatically measured food and water consumption of adult Sdc1+/- and WT animals per day.
(A) Manually measured average food consumption in g/g BW. (B) Average food consumption in g/g BW determined using the PhenoMaster system. (C) Manually measured average water consumption in ml/g BW. (D) Average water consumption in ml/g BW determined using the PhenoMaster system (*p<0.05).
Fig 7.
The activity profile of Sdc1+/- and WT male and female mice measured by the PhenoMaster system within 56 h.
(A, B) Circadian activity of the Sdc1+/- and the WT male and female mice (shaded area indicates light hours 6 a.m.– 6 p.m.). The activity pattern shown was identical for the x, y and/or z axis as well as the activity at the center and at the periphery of the cage.
Fig 8.
The activity of Sdc1+/- and WT male and female mice measured by the PhenoMaster system.
The average activity of two days is shown divided into day and night. (A, B) The average activity on the x and y axis. (C, D) The average activity on the z axis (*p<0.05).
Fig 9.
Central and peripheral activity of Sdc1+/- and WT male and female mice measured by the PhenoMaster system.
The average activity of two days is shown divided into day and night. (A, B) The average activity in the center of the cages. (C, D) The average activity in the periphery of the cages (*p<0.05).
Fig 10.
Energy expenditure of the Sdc1+/- and WT males and females measured by the PhenoMaster system.
(A, B) The energy expenditure profile was measured within 56 h (shaded area indicates light hours 6 a.m.– 6 p.m.). (C, D) The average energy expenditure of two days is shown divided into day and night (*p<0.05).
Fig 11.
Parameters of the examination of the fat depots of the Sdc1+/- and WT animals that were born after normal mating.
(A) Fat weight of inguinal, retroperitoneal, mesenteric and gonadal depots of Sdc1+/- and WT male and female mice in [g] and (B) adjusted for BW. (C, D) The mean adipocyte area [μm2] and the total number of adipocytes per microscopic field of view of the 4 fat depots of Sdc1+/- and WT males and females (*p<0.05).
Fig 12.
Parameters of the examination of the fat depots of the Sdc1+/- and WT animals that were born after vice versa embryo transfer.
(A) Fat weight of inguinal, retroperitoneal, mesenteric and gonadal depots of Sdc1+/- and WT male and female mice in [g] and (B) adjusted for BW. (C, D) The mean adipocyte area [μm2] and the total number of adipocytes per microscopic field of view of the 4 fat depots of Sdc1+/- and WT males and females (*p<0.05).
Fig 13.
Parameters of the examination of each fat depot of the Sdc1+/- and WT animals that were born after normal mating.
(A, C) The mean adipocyte area and number per microscopic field of view isolated from Sdc1+/- and WT male and (B, D) female mice (*p<0.05).
Fig 14.
Parameters of the examination of each fat depot of the Sdc1+/- and WT animals that were born after vice versa embryo transfers.
(A, C) The mean adipocyte area and number per microscopic field of view isolated from Sdc1+/- and WT male and (B, D) female mice (*p<0.05).
Fig 15.
Relative intestinal weight and length differences of adult Sdc1+/- and WT males and females after normal mating.
(A, B) The intestinal weight before (full) and after (empty) removal of the intestinal content. (C, D) The intestinal length is shown for the whole intestine and for the intestinal parts of the small and the big intestine (*p<0.05, **p<0.02).
Fig 16.
Relative intestinal weight and length differences of adult Sdc1+/- and WT males and females after vice versa embryo transfers.
(A, B) The intestinal weight before (full) and after (empty) removal of the intestinal content. (C, D) The intestinal length is shown for the whole intestine and for the intestinal parts of the small and the big intestine (*p<0.05).