Fig 1.
Classical mouse strains typed for 31 P-MLVs and grouped according to known breeding history.
Substrains AKR/N and AKR/J, and substrains C57BL/6 and C57BL/10 carry identical ERVs, whereas the two CBA substrains differ for six ERVs likely due to genetic contamination [37]. The SIM.R Fv1b congenic differs from its SIM progenitor in the presence of 6 ERVs found in the Fv1b donor strain, three of which are linked to Fv1. a+, presence; -, absence; L, solo LTR; H, heterozygous; ND, not done.
Fig 2.
Classical mouse strains typed for 14 Xmvs and grouped according to known breeding history.
a+, presence; -, absence; L, solo LTR.
Fig 3.
Detection of a representative ERV, Pmv7, in 7 inbred strains.
At the top is a diagram of the provirus and cellular flanks with arrows showing positions of 3 primers and the expected products for mice with and without the Pmv7 insertion. At the bottom are PCR test results for 7 strains using the three primers.
Fig 4.
Wild mouse origins and haplotype identities for six ERVs.
The horizontal tracks represent 2–4 megabase chromosome segments surrounding six specific ERV integration sites. ERVs and their genome locations are listed on the left and PCR typing data is on the right. The map locations for each ERV are marked by a vertical black line. Blocks of shared haplotypes are indicated in multiple colors; additional tracks for the three Xmvs show subspecies-specific blocks originating from M. m. domesticus in blue and from M. m. musculus in red.
Fig 5.
Solo LTRs at insertion sites of Pmv16, Xmv41 and Xmv10.
ERV sequences are in red. Flanking sequences of Pmv16 and Xmv41 show target site duplications, highlighted in blue, and these LTRs are at the same sites as the corresponding ERV in B6 mice. There is a Pmv-like solo LTR adjacent to the Xmv10 site in TH mice; NFS is representative of strains that lack this LTR and Xmv10.
Table 1.
Characterization of inbred strains for naturally occurring hematopoietic neoplasms.
Fig 6.
Fv1 variants in classical inbred strains.
A) The diagrams on the left identify protein sequence differences among the 4 inbred strain alleles. On the right are virus titers for E-MLVs sensitive to Fv1 restriction as determined for NIH3T3 (Fv1n), 129 (Fv1nr), DBA (Fv1d), BALB3T3 (Fv1b), and the wild mouse derived SC-1 (Fv1-). Log10 virus titers were determined by the UV-XC overlay assay; each cell line was tested 3–8 times. B) Inbred strains sequenced for Fv1 alleles. Identical Fv1d sequences were found for substrains DBA/2N and DBA/2J.