Fig 1.
The Eu-labeled antigen (ZIKV NS1), Alexa-Fluor-labeled protein L and the serum sample are mixed together in a 384-well microplate. The former binds to the light chains of the antibodies, which if ZIKV-NS1-specific also bind the europium-labeled NS1 antigen. The mixture is exposed to UV light and the close proximity allows the donor and acceptor to elicit the FRET signal, which is measured.
Table 1.
Reference test results for ZIKV-positive samples.
Fig 2.
Class-independent LFRET scores for the first cohort of samples.
Table 2.
Class-independent LFRET test outcomes in different sample categories.
Fig 3.
Class-independent LFRET scores measured from the full panel of ZIKV-positive, heterologous flavivirus-positive (DENV, YFV or TBEV) and flavivirus-negative samples.
Table 3.
Test outcomes in class-specific LFRET tests compared with the reference tests.
Fig 4.
a) Acute-LFRET scores of IgM-positive and -negative samples b) Immunity-LFRET scores of IgG-positive and -negative samples.
Table 4.
Test outcomes when using non-rigorous sample inclusion criteria.
Fig 5.
Euroimmun ELISA vs. class-specific LFRET.
a) Optical density values in Euroimmun IgM ELISA compared to the acute-LFRET scores of the same samples; b) OD values in Euroimmun IgG ELISA test compared to the immunity-LFRET score.
Table 5.
Comparison of the outcomes of the Euroimmun ELISA test with class-specific LFRET test outcomes.
Table 6.
The samples with inconsistent results between Euroimmun ELISA and class-specific LFRET assay.