Table 1.
Solution of 10 mL collagen with additives (added prior to plastic compression).
Fig 1.
A) ATR-FTIR spectra showing secondary structures and B) XRD patterns showing amorphous/crystalline structure for as-made i) Cs and ii) Cp. arrows in the XRD patterns indicate crystalline regions and amorphous regions.
Fig 2.
SEM images of A) Cs and B) Cp particles.
Fig 3.
Size distribution of i) Cp and ii) Cs particles.
Table 2.
Comparison of amino acid composition for fibroin-derived polypeptides, Cp and Cs, obtained from LCMS (Right–from literature, left–as-made).
Table 3.
Comparison of fibroin-derived polypeptides charge (right–from literature, left–as-made).
Fig 4.
XRD patterns of i) HA (ICDD file 00-009-0432) and polypeptides ii) Cs and iii) Cp immersed in SBF.
Fig 5.
SEM of Plastically compressed A) DC, B) 1:10 DC-Cs and C) 1:10 DC-Cp hydrogels immersed in SBF for 7 days (scale bar is 10 μm, arrows indicate particles).
Fig 6.
ATR-FTIR Spectra for A) DC, B) 1:10 DC-Cs and C) 1:10 DC-Cp, gels immersed in SBF at day i) 0, ii) 3, c) 7, iii) 10 and iv) 14.
Fig 7.
Ratio of A) ν3 PO43- and B) ν3 CO32- bands to amide I bands from ATR-FTIR spectra of DC gels containing no additives, Cs and Cp (at a 1:10 ratio to collagen) immersed in SBF (SD, n = 4, p<0.05).
Fig 8.
XRD pattern for A) DC, B) 1:10 DC-Cs and C) 1:10 DC-Cp hydrogels immersed in SBF at days i) 3, ii) 7, iii) 14 (●–HA corresponding to ICDD file 00-009-0432, ■–PE corresponding to ICDD file 00-060-1505).
Fig 9.
ATR-FTIR for A) 1:1 DC-Cs, B) 1:1 DC-Cp, C) 1:2 DC-Cs and D) 1:2 DC-Cp, i) as-made and immersed in SBF for ii) 3 and iii) 7 days.
Fig 10.
Mass of DC, DC-Cs and DC-Cp hydrogels immersed in SBF for A) 0 (as-made), B) 3 and C) 7 days (*—statistically significant, one-way ANOVA, SD, n = 4, p < 0.05).
Table 4.
Mass of hydrogels at day 0 compared to the theoretical values.