Fig 1.
Genital development of Tropidurus torquatus.
A, stage 29; B, stage 33; C, stage 34; D; stage 35; E, early stage 38; F, late stage 38; G, early stage 39; H, late stage 39; I, stage 40; J, late stage 37; K, stage 39; L, stage 40. Legends: acl, anterior cloacal lip; ad, apical disc; gp, genital primordia; gs, genital swelling; hcl, hemiclitoris; hl, hind limb; lp, lobe primordia; pcl, posterior cloacal lip; ss, spermatic sulcus. Scale bar: 0.5mm.
Fig 2.
Hemipenial development of Tropidurus torquatus under SEM analysis.
A, stage 28; B, stage 29; C; stage 30; D, early stage 31; E, late stage 31; F, late stage 32; G, stage 36; H, stage 37; I, stage 38; J, stage 40; K, stage 41; L, stage 42. Legends: ab, anterior bud; acl, anterior cloacal lip; ad, apical disc; adc, apical disc constriction; adp, apical disc primordia; aer, apical epidermal ridge; tr, hemipenial truncus; cop, cloacal opening primordia; cp, calyces primordia; gp, genital primordia; gs, genital swelling; lb, hind limb bud.; pb, posterior bud; pcl, posterior cloacal lip; pl, primordium of lobes; ss, spermatic sulcus; ssb, spermatic sulcus borders; ssc, spermatic sulcus constriction. Scale bar: A-F, 100μm; G-L, 200μm.
Fig 3.
Male and female genital morphology of Tropidurus torquatus. Measurements are indicated for the hemipenial truncus, hemipenial lobes, female proctodeal-urodeal cloacal region (FPUR), and female urodeal corns (FUC).
Right hemipenis in (A) ventral and (B) dorsal view, and female internal genitalia (C). Figure legends: ad, apical disc; gp, genital papilla; int, intestine; lo, left oviduct; os, oviduct sphincter; ro, right oviduct; uc, urodeal corn; uro, urodeal region; pro, proctodeal region; ss, spermatic sulcus. Scale bar: 1mm.
Fig 4.
Adult hemipenis of Tropidurus torquatus under SEM analysis.
(A) calyces, (B) apical disc and (C) reduced papilla. Figure legends: ad, apical disc; cl, calyces; rp, reduced papilla; ss, spermatic sulcus. Scale bar: 100μm.
Table 1.
A comparison of right and left hemipenial trait values of Tropidurus torquatus.
Fig 5.
Regressions of log-transformed traits vs. log-transformed body size (SVL) for genital traits of Tropidurus torquatus.
A-C: regression for hemipenial traits of embryos (A), hemipenial traits of adults (B), and female internal genitalia (C). D-F: comparison of male-female trait regressions for hemipenial truncus vs female proctodeal-urodeal region (D), hemipenial lobes vs. female urodeal corns (E), and hemipenial total length vs. female internal genitalia total length (F). Figure legends: FIGL, female internal genitalia total length; FUC, female urodeal corns; FPUR, female proctodeal-urodeal cloacal region; HL, hemipenial lobes; HPL, hemipenes total length; HT, hemipenial truncus.
Table 2.
Summary of the statistics for ordinary least square (OLS) regression of traits vs. body size (SVL).
Table 3.
ANCOVA results for difference in genitalia between sexes. F-statistics, and P-values for each trait analyzed.