Table 1.
Main clinical and biological characteristics of patients and controls.
Fig 1.
Conventional T cell counts in peripheral blood were similar in sickle cell disease (SCD) patients and controls.
The CD3+ (A-B), CD4+ (C-D) and CD8+ (E-F) cell counts were compared between SCD patients and controls (A, C, E) and between different subgroups (B, D, F). MET: monthly exchange transfusion; VOC: vaso-occlusive crisis.
Fig 2.
IL-13 and IL-17 production by conventional CD4+ T cells was increased in peripheral blood of sickle cell disease (SCD) patients compared to controls.
The frequency of IL-4+ (A-B), IL-13+ (C-D), IL-17+ (E-F) cells among gated CD4+ cells and of IFNγ + (G-J) cells among gated CD4+ (G-H) and CD8+ (I-J) cells were compared between SCD patients and controls. *p<0.05; **p<0.01.
Fig 3.
The number of iNKT cells and their intracellular cytokine production in peripheral blood were similar in sickle cell disease (SCD) patients and controls.
iNKT cell counts (A-B) and frequency of IFNγ+ cells among gated iNKT cells (C-D) from SCD patients and controls.
Fig 4.
MAIT cell counts were increased with enhanced IL-17 production in peripheral blood of sickle cell disease (SCD) patients compared to controls.
MAIT cell counts (A-B) and frequency of IL-17+ (C-D) and IFNγ+ (E-F) cells among gated MAIT cells from SCD patients and controls. *p<0.05; **p<0.01; ***p<0.001.
Fig 5.
Vδ2 γδ T cell counts were increased with decreased IFNγ production in peripheral blood of sickle cell disease (SCD) patients compared to controls.
Vδ2+ cell counts (A-B) and frequency of IFNγ+ (C-D) and IL-17+ (E-F) cells among gated Vδ2+ cells from SCD patients and controls. *p<0.05; **p<0.01.