Table 1.
Collection locations.
Fig 1.
Specimen growth curve for the collection described in this paper.
Fig 2.
Species with four single tentacles.
a,b. Bougainvillia sp. 1PJB (Bin ACR4341): Medusa with four radial canals and four single tentacles; very short unbranched oral tentacles present. Material collected: 16 medusae (11 off Newport Pier, 2 off Balboa at Coral, 3 from Ocean off Dana Point). a, Medusa BIOUG01227-E08. b, Medusa BIOUG01213-C03. c,d,e. Bougainvillia sp. 2PJB (BIN ACR4343): Medusa with four radial canals and four single tentacles, and four very short unbranched oral tentacles. Material collected: 27 medusae (10 off Newport Pier, 12 off Balboa at Coral, one in Delhi Channel, 2 in Ocean off Crystal Cove, one in Ocean off Newport, one in Harbor entrance), one hydroid off Newport Pier. c, Medusa BIOUG01227-D05. d, Hydroid BIOUG01227-A07. e, Manubrium with medusa buds and short unbranched oral tentacles BIOUG19284 H06; f, Bougainvillia sp. 3PJB (BIN ACW4756): Four radial canals, four unbranched tentacles, no oral tentacles. Material collected: One medusa BIOUG01227-G02, off Newport Pier.
Fig 3.
Species with four pairs or triplets of tentacles.
a. Bougainvillia muscus (BIN ACO8375). Medusa with four radial canals, four pairs of tentacles and four short unbranched oral tentacles. Material collected: Two medusae, off Lido Island. These show a close match (>98%) by DNA barcode with specimens of Bougainvillia muscus collected in Skagerrak (Sweden), Roscoff (France) and New Brunswick (Canada). BIOUG01213-H09. b. Bougainvillia sp. 4PJB (BIN ACR4338). Medusa with four radial canals, four pairs of tentacles and four short unbranched oral tentacles. Material collected: 7 medusae, all off Newport Pier. BIOUG01213-G06. c. Bougainvillia sp. 5PJB (BIN ADC0389). Medusa with four radial canals, four pairs of tentacles and four short oral tentacles. Material collected: One medusa off Balboa at Coral. BIOUG19285-A03. d. Bougainvillia sp. 6PJB (BIN ACR 4342). Medusa with four radial canals, four triplets of tentacles and four oral tentacles. Material collected: Three medusae (1 off Newport Pier, two off Lido Island) BIOUG01227-C08. e,f. Bougainvillia sp. 7PJB (BIN ACR 4339): Medusa with four radial canals, four triplets of tentacles, and two unbranched oral tentacles. Material collected: One medusa (off Crew Dock), one hydroid colony (off NAC). a, Medusa BIOUG01213-D09; b, Hydroid BIOUG19284-F02.
Fig 4.
a,b. Amphinema sp. 1PJB (BIN ACR4255): Material collected: four medusae (3 off Newport Pier, one from Harbor entrance), three hydroid colonies, all from off Balboa Pavilion dock. a, Medusa BIOUG19284-G12; b, Hydroid BIOUG19285-D12. c. Amphinema dinema (BIN ACW2490): Material collected: One medusa, off Newport Pier. 100% match of DNA barcode COI with a specimen from Roscoff, France [9]. Medusa BIOUG01227-H02. d,e. Amphinema sp. 2PJB (BIN ACR4184): Material collected: Four medusae (Two off Balboa Pavilion Dock, one from Bayside Marina, one off Newport Pier), three hydroid colonies (two off Newport Pier, one off Balboa Pavilion Dock) d, Medusa BIOUG01227-H10; e, Hydroid BIOUG19285-D07.
Fig 5.
a, Medusa BIOUG19284-C05. b, c, d, This specimen was found in tanks at Orange Coast College, Orange County, CA on 7/19/2018. Hydroid stage not yet found locally.
Fig 6.
a, b. Corymorpha sp. 1PJB (BIN ACR4296). Material collected: 13 medusae (3 at Crew Dock, 3 at Balboa at Coral, 3 at Lido, and one each at NAC, Delhi channel, Newport Pier and Bayside Marina); two hydroids (one at Balboa at Coral, one at Crew Dock). a, Medusa BIOUG19284-H03; b, Hydroid BIOUG19289-A08. c. Corymorpha sp. 2PJB (BIN ADO3522). Material collected: 1 medusa, at Harbor entrance. Medusa CCDB24005-H02. d. Corymorpha sp. 3PJB (BIN ACZ1499). Material collected: 3 medusae (1 at Lido at Genoa, 1 at Crew Dock, 1 at Bayside Marina). Medusa BIOUG19284-E04. e. Corymorpha sp. 4PJB (BIN ACH5005) close DNA match to both C. bigelowi and C. verrucosa. Material collected: 1 medusa (at Lido at Genoa). Medusa BIOUG01213-G01. f. Corymorpha bigelowi (BIN ACH5002). [13]. Material collected: 21 medusae (8 off NAC, 4 from Delhi Channel, 4 off Crew Dock, 2 off Newport Pier, 2 off Lido Island at Genoa, and 1 off Balboa Island at Coral), no hydroids. Medusa BIOUG19284-H07.
Fig 7.
a-e.Coryne eximia (BIN ACR4295). Material collected: 17 medusae (9 at Newport Pier, 5 at Balboa at Coral, 1 each at Crew Dock, Balboa Pavilion dock, and Reef Point CCSP), 8 hydroids (4 at Newport Pier, 2 at CCSP and 1 each at Mussel Rocks and Reef Point, CCSP) and one actinula larva off Newport Pier. These 26 specimens show closely matched DNA barcodes with one another but with no other sequences in BOLD or Genbank. One other (CCDB 24005 C11 off Balboa at Coral) shows a closer match with one specimen from Norway and one from South Africa, and one more (BIOUG01213 F05 off Newport Pier) shows a closer match with a specimen from Chile. a, Medusa BIOUG01227-A09. b, Club Hydroid BIOUG19289-E06. c, Club hydroid BIOUG19289 F01. d, Club hydroid BIOUG19289-E05. The medusa buds arise in the tentacle axils. e, Actinula larva BIOUG01227-H04. f, Coryne uchidai (BIN ACR4087) Club hydroid BIOUG01213-G02: Material collected: 1 hydroid at Lido at Genoa. DNA barcode shows 98.77% match with a specimen from Hokkaido, Oshoro, Japan.
Fig 8.
a-c. Turritopsis dohrnii (BIN ACR4293). Material collected: 8 medusae (2 off NAC, 2 off Balboa at Coral, 3 off Lido at Genoa, one off Balboa Pavilion); one hydroid (off Pavilion Dock). a, Medusa BIOUG01227-C01; b, Club hydroid BIOUG19285-E09; c, Club hydroid BIOUG19285-E09. d,e. Turritopsis nutricula (BIN ACI0369). Material collected: d, One medusa (inverted?) BIOUG19285-G08 (from Newport Harbor entrance); e, Club hydroid CCDB-25431-F11 (Off Newport Pier).
Fig 9.
By-the wind Sailor, Velella velella.
Material collected: One colony, from San Clemente Beach.
Fig 10.
Pink-hearted hydroid, Ectopleura crocea.
a, Hydroid colony (not barcoded); b, Hydroid (not barcoded); c, Hydroid (not barcoded); d, Hydroid BIOUG01227-C07; e, Hydroid BIOUG01213-G03; f, Actinula larva BIOUG01213-E05.
Fig 11.
a. Clytia elsaeoswaldae (BIN ACR4294). Material collected: 2 medusae (off Balboa at Coral) as well as three medusae from Bahia de los Angeles, Mexico. BIOUG19284-D07. b. Clytia hemisphaerica (BIN AAD5403). Material collected: One hydroid BIOUG01227-E11 off Balboa at Coral. c,d. Clytia gracilis (BIN AAD5401). Material collected: One medusa off Newport Pier, one hydroid off Reef Point, Crystal Cove State Park. c, Medusa BIOUG01213-D07; d, Hydroid BIOUG19289-C05. e,f. Clytia sp. 701 AC (BIN AAR9451). Medusa phase: Four tentacles, four developing tentacles, and eight statocysts. Material collected: 3 medusae off Dana Point, one off Lido at Genoa; One hydroid off Balboa at Coral). e, Medusa BIOUG19284-C12; f, Hydroid BIOUG01213-G11.
Fig 12.
a,b. Obelia dichotoma (BIN ACO3910). Material collected: 5 medusae (3 off Newport Pier, one off Balboa at Coral, one off BBSC); 6 hydroids (5 off Newport Pier, one off Reef Point, CCSP). a, Medusa CCDB-25433-B12; b, Hydroid CCDB-24005-A10. Obelia sp. 1PJB (BIN ACR4187). Material collected: 15 medusae (2 offshore from CCSP, 2 at NAC, 7 offshore from Dana Point, 2 from Harbor entrance, 1 off Balboa at Coral and one off PCH bridge); 10 hydroids (4 off Newport Pier, 5 off Balboa at Coral, one from Harbor). c, Medusa BIOUG012213-E12; d, Hydroid BIOUG01213-G10. Obelia geniculata (BIN AAA708). Material collected: One hydroid, off Newport Pier. e, Hydroid BIOUG19284-G10.
Fig 13.
a. Orthopyxis everta (BIN ADE2083). Material collected: One hydroid off Reef Point, Crystal cove State Park. Hydroid BIOUG19289-E01. b. Orthopyxis sp. 1PJB (BIN ADE2082). Material collected: One hydroid off Mussel Rocks, Crystal Cove State Park Hydroid BIOUG19289-D09. c. Orthopyxis sp. 2PJB (BIN ACZ1022). Material collected: One hydroid off Newport Pier. Hydroid BIOUG19284-E01. d. Orthopyxis sp. 3PJB (BIN ADH3873). Material collected: One hydroid from ocean off Newport Beach. Hydroid CCDB-25431-G09.
Fig 14.
a. Close to Eucheilota bakeri (BIN ACW 1912) [25]. 4 statocysts. Material collected: 5 medusae (2 at Newport Pier, 1 at Newport Aquatic Center, 2 at Balboa at Coral). Medusa BIOUG19284-D02 (Ventral). b. Eucheilota sp. (BIN ACR4297). 8 statocysts. Material collected: 3 medusae (1 at Crystal Cove, 2 at Newport Pier). Medusa BIOUG01227-E06. c-f. Eucheilota bakeri (BIN ACR4560) [25]. Material collected: Two hydroid colonies, attached to Bean Clam, Donax gouldii, washed up on the beach under Newport Pier. c, Hydroid BIOUG0213 C11; d, Hydroid BIOUG0213 C11; two medusae (each with 4 statocysts), one from the Harbor entrance, one off Balboa at Coral. e, Medusa CCDB 24005 C03: f, Medusa CCDB-25431-H05.
Fig 15.
Collected from Newport Harbor.
Fig 16.
Liriope tetraphylla: BIN ACH9275.
Material collected: 9 medusae (4 off Newport Pier, 2 off Dana Point, and one each from Balboa at Coral, Newport Harbor, and off CCSP). Medusa BIOUG01227-A02.
Fig 17.
Sequence divergence in the COI Barcode for intraspecific and interspecific comparisons using the data included in this publication.