Fig 1.
Preserved holotype and paratype specimens of Jenynsia sulfurica sp. nov.
Above: holotype CI-FML 7286, male, 21.6 mm SL; below: paratype CI-FML: 7287, female, 32.8 mm SL, from the La Quinta lagoon, Santa Barbara department, Jujuy province, Argentina.
Fig 2.
Live specimens of the sulfide-tolerant species Jenynsia sulfurica sp. nov.
Lateral view of a (A) male and (B) female individual, exhibiting large heads and a prognathous lower jaw with a hypertrophied lip (indicated by arrows) which facilitate respiration at the air-water interface. (C) dorsal and (D) ventral view of a female individual.
Fig 3.
Jenynsia sulfurica can be diagnosed by the (almost) complete absence of scales in the abdominal area.
Ventral surface photograph of (A) IBIGEO-I 465, female, 25.4 mm; and (B) CI-FML 7287, female, 24.0 mm SL, from the La Quinta lagoon, Santa Barbara department, Jujuy province, Argentina.
Fig 4.
Schematic representation of head squamation and of the head lateral-line sensory system of Jenynsia sulfurica.
Table 1.
Morphometric measurements taken in Jenynsia sulfurica n. sp.; N = 30 individuals including the holotype; SD equals standard deviation.
Fig 5.
Hydrological map of South America.
Red rectangle highlights the Province of Jujuy, where the type locality of Jenynsia sulfurica is indicated by a red dot. (Digital map from catalog.data.gov, U.S. Government Works subject to no copyright).
Fig 6.
Photographs of the habitat of Jenynsia sulfurica.
(A) the lagoon and (B) the sulfide spring which drains into the lagoon. (C) Pond of more than 10 cm water depth where adults of J. sulfurica were found and (D) small pond of 2 cm water depth inhabited by juveniles of J. sulfurica.
Fig 7.
Haplotype network based on statistical parsimony of mitochondrial cox1 gene.
Fig 8.
Topologies of most equally parsimonious trees and GC values.
(A) equal weights and (B) implied weighting with supports measured under K = 8. Unsupported nodes are shown as collapsed. Families and outgroups are depicted for the first comprehensive analysis, combining morphological and molecular data.