Fig 1.
Correlations between CSF unmodified IAPP and Q-albumin, CSF albumin and AD biomarkers.
Scatter plots demonstrating significant correlations between logarithmic transformed values of unmodified CSF IAPP and logarithmic transformed values of unmodified plasma IAPP (A), logarithmic transformed values of Q-Alb (B), logarithmic transformed values of CSF albumin levels (C), logarithmic transformed values of CSF t-tau levels (D) and logarithmic transformed values of CSF p-tau levels in cognitively healthy individuals (NCs)(E). Data was analyzed with Pearson correlation test. * Significant correlation at p<0.05 level.
Table 1.
Data on individuals included in the study.
Fig 2.
Western blot analysis of islet amyloid polypeptide (IAPP) preparations and cerebrospinal fluid (CSF) and correlations between total IAPP, Q-albumin, CSF albumin and AD biomarkers.
Immunoblotting with T4149, T4157 recognized low molecular weight (A133,) IAPP in wells loaded with monomeric preparations of IAPP (mIAPP), whereas A133 only recognized the LMW after membrane boiling (A). All three antibodies also recognized several bands with various molecular weights (ranging from 10 kDa to over 300kDa) in the pre-aggregated preparation of IAPP (aIAPP) and a 120 kDa in CSF (A). The A133 detected additional bands in CSF both before and after membrane boiling (A). Scatter plots in (B-D) demonstrate correlations between total CSF IAPP and logarithmic transformed values of Q-albumin in NC (B), AD patients (C) and patients with both AD and T2D (D). Scatter plots in (E-G) demonstrate correlations between total CSF IAPP and logarithmic transformed values of CSF albumin in NC (E), AD patients (F) and patients with AD and T2D (G). Data was analyzed with Pearson correlation test. * Significant correlation at p< 0.05. ** Significant correlation at p<0.001. *** Significant correlation at p< 0.0001.
Fig 3.
Correlations between total plasma IAPP and AD biomarkers.
Scatter plots demonstrate correlation between levels of total plasma IAPP and logarithmic transformed values of CSF t-tau in NCs (A) and a trend towards a correlation in patients with AD+T2D (B). Scatters plots also demonstrate a positive correlation between levels of total plasma IAPP and logarithmic transformed values of CSF p-tau in NCs (C) as well as a negative correlation between levels of total plasma IAPP and logarithmic transformed values of CSF Aβ42 in AD patients (D). Data was analyzed using Pearson correlation test. * Significant correlation at p<0.05.