Fig 1.
Workflow of study design and statistical analyses for biological replication.
Table 1.
Characteristics of NEO reproducibility sub-population.
Fig 2.
The ICC score distributions on biological reproducibility by (A) comparisons between fasting and postprandial, and (B) short- and long-term visits.
Metabolites are ranked ascendingly by fasting (top) and short-term ICC scores (bottom). The lower corner represents the density plot of ICC score distributions from fasting and postprandial samples (top) or short- and long-term samples (bottom) separately, with the p-value derived from Wilcoxon two-sided test. The dash lines correspond to the median ICC scores among fasting (blue) and postprandial (green) samples (top) or short- (blue) and long-term (green) samples (bottom).
Table 2.
Technical and biological reproducibility of selected metabolic biomarkers reported in the literature.
Fig 3.
The ICC score distributions for biological reproducibility by comparisons between fasting/postprandial state, stratified on time interval between visits (short-/long-term).
Metabolites are ranked ascendingly by fasting short-term (blue dots). The lower corner contains a density plot of ICC score distributions from fasting short-term (blue), fasting long-term (green), postprandial short-term (purple) and postprandial long-term (orange) separately. The dash lines correspond to the median ICC scores among four scenarios.
Fig 4.
Decomposition of variance for each metabolite.
Metabolites are ordered by commercial clusters. Fasting/postprandial state (F/P) corresponds to fasting (F) and postprandial; Time interval (S/L) stands for short- (S) and long-term.