Fig 1.
Schematic representation of the experimental design.
LFD, Low-Fat Diet; HFD, High-Fat Diet; ABS, Antibiotics; FMT, Fecal Microbiota Transplantation.
Fig 2.
A) Measurement of body weight during the 14 weeks of study and B) the percentage of body fat measured at weeks 1, 9, 11 and 14. *p<0.05, calculated using ANOVA.
Fig 3.
A) PCA of the family OTU abundance and the proteins that were identified that shows the separation between the LFD and HFD groups. The first two components are shown along with the percent variance that is explained by each. The points correspond to the individual samples.
Table 1.
Families with significant differences in abundance between the LFD and HFD groups. Metagenomics analysis.
Table 2.
Proteins significantly up- or downregulated between the LFD and HFD groups.
Metaproteomics analysis.
Fig 4.
A) Differences in the actual OTU abundances among the different groups at the phylum taxon levels. B) PCA of the differences between the groups treated with and without ABS. The first two components are shown along with the percentages of variance that they explain. The points correspond to individual samples.
Table 3.
Families with significant differences in abundance between the diet-only groups and the respective diet-plus-ABS groups.
Metagenomics analysis.
Fig 5.
A) PCA of OTU abundance. The first two components are shown along with the percentage of variance that they explain. The points correspond to individual samples. B) Hierarchical clustering analysis of the three significant families in the LFD, HFD and FMT groups.
Table 4.
Families with significant differences in abundance based on ANOVA between the LFD, HFD and FMT groups.
Metagenomics analysis.
Fig 6.
A) PCA of up- and downregulated proteins showing the separation between the three groups. The first two components are shown along with the percentages of variance that they explain. The points correspond to individual samples. B) Percentages of proteins that represent the 15 most abundant protein functions according to Gene Ontology (GO) terms in the LFD, HFD and FMT groups. C) Hierarchical clustering analysis of 21 significant proteins in the LFD, HFD and FMT groups.
Table 5.
Proteins significantly up- or downregulated between the LFD, HFD and FMT groups. Metaproteomics analysis.
Fig 7.
Significant correlations between families and proteins in the LFD, HFD and FMT groups.