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Fig 1.

Historical timeline of 16th-century Italian herbaria.

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Fig 2.

The Rome herbarium puzzle.

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Fig 3.

The En Tibi herbarium (A) and specimens of Anemone hortensis (B) and Tulipa sylvestris (C).

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Fig 4.

Botanical similarity between the En Tibi and five contemporary herbaria.

100%: exact similarity; 75%: large similarity; 50% partial similarity; 25%: vague or coincidental similarity; 0%: similarity. The similarity with the Rome herbarium is estimated for Erbario B and at a second step combined Erbario B and Erbario C (See Fig 2 and S1 Appendix).

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Fig 5.

En Tibi (A) vs. Rome herbarium (B), and Cibo painting (C). See also S4 Appendix.

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Fig 6.

Ranunculus arvensis (left) and R. gracilis (right) in the En Tibi (A) and Rome herbarium (B).

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Fig 7.

Salix x rubra in the En Tibi (A) and Rome herbarium (B). Galls are indicated with arrows.

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Fig 8.

Scrophularia canina in the En Tibi (A) and Rome herbarium (B).

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Fig 9.

Examples of handwriting of the En Tibi, Rome herbarium and candidate makers.

En Tibi index and content (A); Erbario B index (B); Erbario C index (C); Gherardo Cibo (D); Francesco Petrollini (E).

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Fig 10.

Hammer-and-anvil watermark.

Type S (short-tip; left) and type L (long-tip; right).

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Fig 11.

Parchment fragments found during the En Tibi’s restoration.

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