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Fig 1.

Minimal Inhibitory Concentration [MIC] and Minimal Biofilm Eradication Concentration [MBEC] of gentamicin towards planktonic [P] and biofilm-forming [B] cells of P.aeruginosa [P.a], S.aureus [S.a.], K.pneumoniae [K.p.].

Asterisks show statistical significance [K-W test, p<0.05] between results obtained for P vs. B of particular microbial species.

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Fig 1 Expand

Fig 2.

P.aeruginosa biofilm formed on HA surface.

Upper part of picture–multilayer form of mature P.aeruginosa biofilm [Magn.2500x]; lower part of pictureP.aeruginosa biofilm-forming cells [Magn.20000x]. Zeiss Evo Ma SEM.

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Fig 2 Expand

Fig 3.

Comparison of Minimal Biofilm Eradication Concentration [MBEC] of gentamicin against biofilm formed on hydroxyapatite [HA] or polystyrene [Poly] surfaces by P.aeruginosa [P.a], S.aureus [S.a.], K.pneumoniae [K.p.] in 24-well plates.

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Fig 3 Expand

Fig 4.

Comparison of the number of S.aureus biofilm-forming cells after gentamicin sponge application [GS] to the number of biofilm-forming cells of untreated control samples [C].

8,24,48 –stand for the hours of incubation of the analyzed sample within the sponge. For the Reader’s convenience, a logarithmic scale was applied here. All the differences between the respective control and analyzed biofilm samples are statistically significant (M-W test, p<0.05).

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Fig 4 Expand

Fig 5.

Comparison of the number of P.aeruginosa biofilm-forming cells after gentamicin sponge application [GS] to the number of biofilm-forming cells of untreated control samples [C].

8,24,48 –stand for the hours of incubation of the analyzed sample within the sponge. All the differences between the respective control and analyzed biofilm samples are statistically significant (M-W test, p<0.05).

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Fig 5 Expand

Fig 6.

Comparison of the number of K.pneumoniae biofilm-forming cells after gentamicin sponge application [GS] to the number of biofilm-forming cells of untreated control samples [C].

8,24,48 –stand for the hours of incubation of the analyzed sample within the sponge. Statistically significant differences between the respective control and analyzed biofilm samples are marked with an asterisk (M-W test, p<0.05).

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Fig 6 Expand