Table 1.
Diet composition based on caloric distribution.
Table 2.
Diet fatty acids composition.
Fig 1.
The effect of low lard fat-based (LFD), high lard fat-based (HFD) and standard chow (SCD) diets on cardiac structure and histology.
(A) Hematoxylin and eosin-stained ventricle mid-region heart cross sections and quantitative morphometry of septal (SWT) and posterior (PWT) wall thickness, left ventricular chamber diameter (LVCD), ratio of left ventricular wall thickness (WT) to LVCD, and left ventricular mass (LVM) from the LFD-, HFD- and SCD-fed mice. (B) Representative histological sections stained with hematoxylin and eosin (40x) and quantification of cardiomyocyte diameter. (C) Representative slides stained with Masson’s trichrome (10x); fibrosis was evaluated as a percentage of blue-stained pixels (collagenous tissue) to the sum of blue and red pixels (total tissue area) x 100% using Image J software. Data presented as mean ± SD, n = 7 for the SCD, n = 7 for the LFD, and n = 7 for the HFD. LV–left ventricular; RV–right ventricular.
Table 3.
Biometric data and calorie intake of mice fed with standard chow, low lard fat- and high lard fat-based diets.
Fig 2.
The effect of low lard fat-based (LFD), high lard fat-based (HFD) and standard chow (SCD) diets on activity of mitochondrial OXPHOS complexes and citrate synthase in ventricular tissue.
The functional activity of OXPHOS complexes and citrate synthase was measured spectrophotometrically as described in Material and Methods and normalized to citrate synthase activity (nmol/min/citrate synthase activity). The citrate synthase activity was expressed as nmol TNB/min/mg protein. Data presented as mean ± SD, n = 7 for the SCD, n = 7 for the LFD, n = 7 for the HFD.
Fig 3.
Malondialdehyde level in ventricular tissue from low lard fat-based (LFD), high lard fat-based (HFD) or standard chow (SCD) diet-fed mice.
Malondialdehyde (MDA) concentration was determined by a thiobarbituric acid assay in ventricular tissue homogenates as described in Material and Methods. Data presented as mean ± SD, n = 5 for each group.