Table 1.
Target and reference genes, primers sequences and melting temperatures for qPCR analysis.
Fig 1.
Heatmap representing the abundance of differentially expressed genes (rows, FDR < 0.01, fold change > 2) in each cDNA library (columns).
Dendogram shows that female and male O. maya white body samples were clustered in different groups. Libraries: MPRE = male pre-copula, MPOS = male post-copula, FPRE = female pre-spawning, FPOS = female post-spawning. Values represent fold change in log2.
Fig 2.
Top GO terms (biological process, level 3) from the reference transcriptome of O. maya white body.
The categories with more transcripts assigned are shown.
Table 2.
Transcripts and their encoded putative proteins from O. maya white body, classified in the immune process GO category.
Table 3.
Hematopoietic fingerprint proteins [52], detected in the reference transcriptome of O. maya white body.
Table 4.
KEGG pathways enriched by the differentially expressed transcripts between female and male WB of O. maya.
Table 5.
Top-20 enriched GO (fisher exact test, FDR < 0.05) in female and male O. maya white body.
Table 6.
Representative genes for the enriched GO (biological process, BP) in female (F) and male (M) white bodies.
Fig 3.
Phylogenetic relationship of the putative neuropeptide-coding transcripts from the O. maya white body transcriptome.
The percentage of replicate trees in which the associated sequences clustered together in the bootstrap test (200 replicates) are shown next to the branches (values above 40% are shown). The analysis involved 90 nucleotide sequences and a total of 255 positions in the final dataset. The subtree including the closer sequences to the putative transcripts are shown. Above: Nucleotide-based analysis. Below: Codon-based analysis.
Fig 4.
Relative expression levels of the putative neuropeptide transcript TRINITY_DN12598_c0_g1_i6 analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 5.
Relative expression levels of corticotropin-releasing hormone receptor 2 (CRHR2) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 6.
Relative expression levels of estrogen receptor (ESR1) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 7.
Relative expression levels of estradiol 17-beta-dehydrogenase 8 (HSD17B8) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 8.
Relative expression levels of ATP-dependent RNA helicase DDX4 (VASA) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 9.
Relative expression levels of piwi-like protein 1 (PIWIL1) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 10.
Relative expression levels of zonadhesin (ZAN) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.
Fig 11.
Relative expression levels of C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9) analyzed via qPCR.
Values represent the fold change (log10) of each target vs the reference genes. Reference genes: HNRNPD and VATD. Samples: FWB = female white body, FH = female heart, MWB = male white body, MH = male heart, MT = male testis. The 95% confidence interval of each group is shown and the significant differences among groups are represented with different letters.