Fig 1.
Wild-type (left) and mutant (right) leaves phenotype.
Bar = 5 cm.
Fig 2.
Pigment contents in wild-type and mutant leaves.
(A) Schematic view of the Chl biosynthetic pathway. Urogen III (in red) at the beginning of the pathway shows a significant increase in yellow leaves. CHLG/P illustrates the gene encoding protein catalyzing the reaction of the precursors. A green box represents each component that is significantly decreased or down-regulated in the yellow leaves. Coprogen III had no significant difference between green and yellow leaves. (B) Comparison of the relative content of Chl precursors and photosynthetic pigments between wild-type and mutant. (C) Comparison of the flavonoid contents between wild-type and mutant leaves. Asterisks indicate: (*) P ⩽ 0.05, (**) P ⩽ 0.01. Abbreviations are: Urogen III = uroporphyrinogen III; Coprogen III = coproporphyrinogen III; Proto IX = protoporphyrin IX; Mg-Proto IX = Mg-protoporphyrin IX; Pchlide = protochlorophyllide; Chlide a = chlorophyllide a; Chl a = chlorophyll a; Chl b = chlorophyll b; and Caro = carotenoid.
Table 1.
Summary of the sequencing and mapping results.
Fig 3.
Number of specific and shared genes between wild-type and mutant leaves.
Fig 4.
The DEG function classification in wild-type and mutant leaves.
X-axis displays the number of genes. Y-axis is broken into three categories: biological process, cellular component and molecular function with enrichment details within each category.
Fig 5.
Enriched KEGG pathways of DEGs.
X-axis displays the Rich factor. Y-axis names the KEGG pathways. Dot size corresponds to the number of DEGs in the pathway with smaller dots having a lower gene number than larger dots. Dot color indicates P value with red having a high P value and blue having a low P value.
Fig 6.
Chl biosynthetic pathways at the transcript level.
Red notates up-regulated genes and green marks down-regulated genes.
Table 2.
DEGs involved in Chl and flavonoid biosynthesis in mutant transcriptome.
Fig 7.
RT-qPCR vs. RNA-seq analysis of 8 DEGs in mutant leaves.
X-axis is the Gene ID. Y-axis is the fold change in mutant leaves relative to that in wild-type leaves (Log2(FC)).