Fig 1.
Hierarchical sample clustering bar-chart showing 20 most abundant genera.
Sample names start with C or O (control or oregano), number (rooster 1, 2 or 3) and replicate code (A or B for 2 replicates).
Fig 2.
High effect of biological microbiota donor.
Panel A shows Bray-Curtis PCoA plot separating samples by the rooster donor rather than oregano; panel B shows linear discriminant analysis (LDA) effect size method showing genera likely responsible for difference between the rooster’s microbiotas.
Fig 3.
DeSeq2 differentially abundant taxa.
Table with all significant OTUs is provided in S1 File.pdf, Table B.
Fig 4.
Concentrations of SCFA in control and oregano supplemented cultures.
One-way Anova was used to determine significance. There were two SCFA significantly increased, which were AA (p = 6.6e-4) and BA (p = 6.3e-3).
Fig 5.
SCFA: AA and BA spearman correlations of concentration and abundance.
Fig 6.
Heatmap (A) and a network (B) showing 20 most abundant genera relationship with SCFA and CAR. Both figures are based on Spearman correlation.
Table 1.
The concentration of CAR in cultures shown in mmol/L.
CAR concentrations were determined by GCMS analysis.