Fig 1.
Objective workflow (pipeline) used in the context of integrative taxonomy to delimitate Characins in ichthyological collections.
Black and grey squares represent the possible rearrangements for integrative taxonomy. Full match, by which the three molecular methods generated the same grouping; Partial Match by which at least two of the molecular methods generated the same grouping; and Discordant, by which all three molecular methods led to a different result.
Fig 2.
Bayesian phylogenetic tree of Eretmobrycon, Bryconamericus, Hemibrycon and Knodus obtained with COI data.
The red asterisks in the node branches represent a posterior probability higher than 95%, and the red terminal branches in the tree show the transition point from Yule to a coalescent branching process in the analysis of all sequences as estimated by the single-threshold model in the GMYC test. The first column represents monophyletic clusters recovered by GMYC analysis, and the second and third columns correspond to the status of the delimitation of OTUs by PTP and ABGD. Full match, Partial Match and Discordant categories between the three molecular methods are represented by black and grey squares. OTUs recovered with all methodologies including initial morphological assessment are indicated with a black circle. Identification errors and suggested changes or taxonomic confirmations (to genus and/or species levels) are highlighted in red letters. The red asterisk in the nodes is indicating PP ≥0.95. The letter T indicates the type species of the genus.
Table 1.
Number of specimens reassigned to other genera after ED and morphological reassessment.
Table 2.
New classification suggested and confirmed by delimitation species.