Fig 1.
Putative biosynthetic pathways beginning from calonectrin (CAL) leading to different F. graminearum trichothecenes discussed in this study.
7,8-dihydroxycalonectrin (7,8-hydroxyCAL); 7-hydroxycalonectrin (7-hydroxyCAL); 3,15-diacetyldeoxynivalenol (3,15-ADON); 15-acetyldeoxynivalenol (15ADON); 3-acetyldeoxynivalenol (3ADON); deoxynivalenol (DON); 3ANX (NX-2 toxin); NX (NX-3 toxin).
Table 1.
Genotype and chemotype of the strains tested.
Fig 2.
Comparison of the MS/MS spectra of a 3ANX standard at (a) 20 NCE and (b) 40 NCE with the putative 3ANX (c,d) detected culture filtrates of strain DAOMC 251912.
Fig 3.
LC-MS/MS extracted ion chromatogram of (a) 3ANX standard with the (b) 3ANX detected in culture filtrates of DAOMC 251907.
Fig 4.
MS/MS product ion spectra of protonated (a) 3ADON and (b) 15ADON. All the major product ions produced by MS/MS of 3ADON are also produced by 15ADON at different proportions.
Fig 5.
LC-MS/MS chromatograms of standard mix containing (a) 4ANIV, (b) NIV, (c) 15ADON and 3ADON, (d) DON, (e) 3ANX and (f) NX toxin monitored by settings in Table 2.
Table 2.
Optimized MS/MS settings for targeted analytes.