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Fig 1.

The proposed framework.

The Block diagram of the proposed framework for muscles/fat segmentation and quantification based on MRI 3-D volumes.

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Fig 2.

An Example for applying LCDG algorithm MRI 3-D volumes.

LCDG algorithm output on (a) exemplary 3D FS-MRI image data; (b) probability density functions of the image voxels in Fig 2A, as determined empirically, and as approximated via LCDG using two dominant DGs; (c) the deviations (standard and absolute) between the empirical and estimated marginal probability density functions in Fig 2B; (d) LCDG algorithm output on the dominant and subordinate DGs in the image data in Fig 2A; (e) the final estimated LCDG model of the empirical density function; and (f) the final LCDG output of the conditional probability density functions of light tissue (muscle) and dark tissue (fat) intensities and the empirical density function.

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Fig 3.

Segmentation accuracy measures.

(a) In the segmentation quality measurements, there are 4 regions to be considered as: True positive (TP), false positive (FP), true negative (TN), and false negative (FN). (b) The calculation of the HD between the red line X and the blue line Y.

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Fig 4.

Examples for the utilization of LCDG to segment the soft tissue volumes.

(a) From left to right: gray scale MR images for FS+WS, WS and FS; (b) From left to right: binary mask of total thigh area, total fat and total muscle area; (c) From left to right: steps for segmenting the bone and bone marrow; (d) 3-D representations of the segmentation results for SCI (left) and ND (right) thigh; Grey: Muscle area, Yellow: SAT, Blue: IMAT, Red: bone.

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Table 1.

Accuracy measures for adipose tissue and total muscle area using LCDG method.

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Table 1 Expand

Fig 5.

Examples of muscle group segmentation algorithm for four SCI subjects.

(a) original cross sectional MR image; (b) automatic segmentation of muscle groups: blue area is extensor, red is flexor and yellow presents the medial compartment; (c) manually segmented muscle groups (cyan lines) overlaid on automatic segmentation for comparison; and (d) 3-D representation of automatic segmentation of muscle groups: blue volume is extensor, red is flexor and yellow presents the medial muscle group.

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Table 2.

Accuracy measures for segmenting three muscle compartments using the proposed method, ANTs and STAPLE.

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Table 2 Expand

Fig 6.

The boxplot representation of the calculated volumes and ratios for manual (black) and automatic (red) segmentation results.

(a) Extensor volume; (b) Flexor volume; (c) Medial volume; (d) IMAT volume; (e) SAT volume; and (f) Total muscle volume.

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