Fig 1.
Morphology and cell density of SHED and HFSCs cultured in different media combinations.
The cell morphology of SHED and HFSCs in the media combinations; DMEM-KO+10% FBS, STK2+2% FBS+STK2 at Day 3 of passage 3, observed under 10× magnification. *A- elongated spindle shape, B-short round shape, +-low cell density, ++- moderate cell density, +++- high cell density.
Fig 2.
The population doubling times and viability of SHED and HFSCs cultured in the different media combinations from passage 2 to passage 5.
(a) The population doubling times of SHED and HFSCs cultured in the different media combinations from passage 2 to passage 5. *The same alphabet indicates a significant difference (p<0.05). There was no statistically significant difference between the population doubling times between SHED and HFSC. (b)The percentage of viability of SHED and HFSCs cultured in media combinations; DMEM-KO+10% FBS, STK2+2% FBS and STK2 from passages 2 to 5.
Table 1.
The mean (±SE) hair regulatory paracrine factor concentrations in ng/106cells for SHED cultured under the different culture media combinations; DMEM-KO+10% FBS, STK2+2% FBS and STK2 at passage 3 and passage 4.
Table 2.
The mean (±SE) hair regulatory paracrine factor concentrations in ng/106cells for HFSCs cultured under the different culture media combinations; DMEM-KO+10% FBS, STK2+2% FBS and STK2 at passage 3 and passage 4.
Fig 3.
Representative photomicrographs of ICR mouse skin samples treated with SHED-CM and HFSC-CM.
Original magnification 40×. Stained with H&E.
Fig 4.
Percentage of hair follicles at each hair growth stage on day 1 and day 3, post CM treatment under in vitro conditions.
The percentage of hair follicles transferred from the telogen-stage to the subsequent stages of hair growth following the CM treatment. *The same alphabet indicates a significant difference (p<0.05) aa-Significant increase in anagen stage bb-Significant decrease in catagen stage cc-significant decrease in telogen stage D = Day P = Passage.
Fig 5.
Number of days taken for the appearance of dark patches and almost complete hair coverage with the corresponding paracrine factor profiling of the CM prepared in STK2-serum free media.
The number of days taken for the appearance of dark patches and almost complete hair coverage in C3H/HeN mice when treated with three subcutaneous injections of 100μl of SHED-CM (n = 3), HFSC-CM (n = 3) and STK2 (n = 3) at three day intervals and the untreated C3H/HeN mice (n = 2). The corresponding cytokine profiling for the donor from which the CM was prepared in also indicated. * Standard error = 0, # Standard error = 1 and D = Donor.
Fig 6.
Time duration taken for the appearance of dark patches and almost complete coverage of hair upon treatment with SHED-CM and HFSC-CM.
(a) The number of days taken for the appearance of dark patches in C3H/HeN mice (n = 9) when treated with three subcutaneous injections of 100 μl of SHED-CM and HFSC-CM at three day intervals (b) The number of days taken for almost complete hair coverage of the C3H/HeN mice (n = 9) when treated with three subcutaneous injections of 100μl of SHED-CM and HFSC-CM at three day intervals. * indicates a significant difference (p<0.05).