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Table 1.

Nutritional composition of loose-hay and pelleted-hay alfalfa diets, as well as in situ rumen digestibility.

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Fig 1.

Particle size distribution of in diet treatment biomass, and percentage of residual feed particles in feces in wethers on a loose-hay (Hay) and pelleted (Pellet) alfalfa diet.

Intake was averaged across 3-day blocks over a 14-day period.

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Fig 1 Expand

Table 2.

Weight, intake, and efficiency values for wethers on loose-hay diet and pelleted-hay alfalfa diets.

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Table 2 Expand

Table 3.

Blood serum parameters for wethers fed either a loose alfalfa hay or pelleted alfalfa diet.

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Fig 2.

Observed bacterial richness (A) and Shannon diversity (B) in the rumen of wethers on loose-hay or pelleted-hay alfalfa diets. Significance was determined at p < 0.05, by linear mixed model for observed SVs and Conover test for Shannon diversity, with sheep ID as a fixed effect.

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Fig 2 Expand

Fig 3.

Distance-based redundancy analysis of Bray-Curtis Distance between rumen bacterial communities in wethers receiving loose-hay or pelleted-hay alfalfa diets.

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Fig 3 Expand

Fig 4.

Non-metric multidimensional scaling (NMDS) plot of unweighted Jaccard distance between rumen bacterial communities in wethers receiving loose-hay or pelleted-hay alfalfa diets.

Ellipses represent treatment weeks. Lowest stress = 0.0649, R2 = 0.9958.

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Fig 4 Expand

Fig 5.

Discriminatory rumen bacterial sequence variance by treatment group for wethers receiving loose-hay or pelleted-hay alfalfa diet treatments.

Significance (p < 0.05) determined by binomial test.

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Fig 5 Expand

Fig 6.

Correlations between the 50 most abundant rumen bacteria and lamb growth, serum parameters, and treatments.

Only significant (P < 0.05) Spearman’s correlations are reported.

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Fig 6 Expand

Table 4.

Serum factors significantly correlated (Spearman’s) with weight in lambs.

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Table 4 Expand