Fig 1.
ChIP-seq comparison between Santa Cruz (sc-543), Millipore (06–935) and Abcam (ab3575) antibodies.
A) Venn diagram showing the overlap between ERα binding sites for Santa Cruz (sc-543), Millipore (06–935) and Abcam (ab3575) antibodies in MCF7 cells. B) Pearson's correlation between each replicate of all three antibodies in MCF7 cells. C) Top: De novo motif analysis of ERα binding sites using MEME. Bottom: Heatmap of total number of ERα binding sites identified in both technical replicates of MCF7, and in all three biological replicates for MDA-MB-231, respectively. D) Examples of ERα- bound regions. Tag densities are shown as reads per million.
Fig 2.
Comparison of RIME data between Santa Cruz (sc-543), Millipore (06–935) and Abcam (ab3575) antibodies.
A) Protein sequence coverage of ERα achieved by the use of Abcam (ab3575), Millipore (06–935) and Santa Cruz (sc-543) antibodies in RIME. B) PCA plot of known ERα interactors (n = 319, BIOGRID and STRING databases) for the four different RIME pull-downs. C) Hierarchical clustering of the scaled intensities of known ERα interactors from BIOGRID and STRING databases (n = 319). D) Hierarchical clustering of well-characterized ERα interactors.