Table 1.
Sampling times and tank distribution for each group.
Table 2.
Parameters of the MS/MS detector.
Table 3.
Monitored ion masses.
Table 4.
Adjustment, type of distribution and parameters of the Kolmogorov-Smirnov test.
Table 5.
LD50 bacterial counts of inoculum dilutions.
Table 6.
Distribution of isolates by year, host species and geographical area.
Fig 1.
Distribution of the Minimum Inhibitory Concentration values of 87 strains of Piscirickettsia salmonis versus florfenicol.
Distributions obtained through the ECOFFinder (a) and NRI (b) programs. Bars represent the gross count of the number of isolates in each concentration of antimicrobial; lines represent the curves of best fit for the distribution of WT strains.
Table 7.
Minimum inhibitory concentration (MIC) and epidemiological cut-off points (COWT) for the total strains of P. salmonis analyzed against Florfenicol.
Fig 2.
Average plasma concentrations (μg/mL) by sampling time (hours) and dose: 10, 15 and 20 mg/Kg.
Different letters represent statistical differences between groups (p<0.05).
Table 8.
Average pharmacokinetics of florfenicol parameters by the trial dose.
Table 9.
PK/PD index (AUC0-24h/ MIC) for doses 10, 15 and 20 mg/ Kg.
Table 10.
Statistical parameters and probability of reaching a target reference point greater than 125 (TRP>125) AUC20 mg/MICn.
Table 11.
Integration of pharmacokinetic and pharmacodynamic variables (PK/PD) using the Montecarlo simulation.
Fig 3.
Cumulative mortality (%) in Atlantic salmon after P. salmonis challenge in dilutions of 1/10, 1/100, 1/1000 and 1/10000. TK: Tank.
Table 12.
RT-PCR SRS results at days 3 and 6 post challenge with P. salmonis.
Fig 4.
Specific Feed Rate (%) by date of experiment.
Challenge group: Tanks 2 and 3; Control (+) (without medication): Tanks 5 and 6; Control (-) (without challenge): Tank 9. TK: Tank; SFR: Specific Feed Rate. For more information, see S4 Table.
Fig 5.
Cumulative mortality (%) by date of experiment.
Challenge group: Tanks 2 and 3; Control (+) (without medication): Tanks 5 and 6; Control (-) (without challenge): Tank 9. For more information, see S5–S7 Tables.
Table 13.
RT-PCR SRS results from necropsy of mortality of Atlantic salmon challenge with P. salmonis during the experiment.
Table 14.
RT-PCR SRS results from necropsy of euthanized fish (Atlantic salmon) after the experiment.