Fig 1.
Morphology of endophytic fungus AE1 under light microscope.
Fig 2.
Neighbor joining phylogenetic tree on the basis of 28S rDNA (D1/D2 region) sequence of AE1.
Table 1.
Antibacterial activity of ethyl acetate extract of AE1 by disc diffusion method.
Table 2.
Effect of ethyl acetate fraction of AE1 on CFUs of pathogenic bacteria.
Fig 3.
Mode of action of ethyl acetate fraction of AE1 on bacterial growth.
Fig 4.
Effect of ethyl acetate fraction of AE1 on the morphology of pathogenic bacteria found during SEM studies: A- B. subtilis (untreated); B- B. subtilis (treated); C- L. monocytogenes (untreated); D- L. monocytogenes (treated); E- S. aureus (untreated); F- S.aureus (treated); G- S. typhimurium (untreated); H- S. typhimurium (treated); I- E. coli (untreated); J- E. coli (treated).
Fig 5.
Release of intracellular materials from pathogenic bacterial cells upon treatment with EA fraction of AE1: A- release of DNA; B- release of protein; C-leakage of K+ ion.
Fig 6.
Profile of central carbohydrate metabolic enzymes in pathogenic bacteria upon treatment with ethyl acetate extract of AE1: A- B. subtilis; B- L. monocytogenes; C-S. aureus; D- E. coli; E- S. typhimurium.
Fig 7.
Thin layer chromatographic analysis of ethyl acetate fraction of AE1.
Fig 8.
Zones of inhibition produced by compounds derived from TLC plate, against pathogenic bacteria A- B. subtilis; B- L. monocytogenes; C- S. aureus; D- E. coli; E- S. typhimurium.
Fig 9.
Chemical structures of twelve different compounds identified from band-C of EA fraction of AE1 by GC-MS analysis as mentioned in Table 3.
Fig 10.
Chemical structures of five different compounds identified from band-D of EA fraction of AE1 by GC-MS analysis as mentioned in Table 4.
Table 3.
Compounds identified from band-C of EA fraction of AE1 by GC-MS analysis.
Table 4.
Compounds identified from band-D of EA fraction of AE1 by GC-MS analysis.