Table 1.
Baseline characteristics and parameters assessed before and after initiation of COH in study participants undergoing IVF.
Table 2.
Hormonal parameters measured in follicular fluid of study participants on the day of oocyte pick up (d-OPU).
Fig 1.
Schematic representation of the workflow of glycoprotein enrichment.
By iTRAQ-based strategy employed for the comparison of follicular fluid proteome from healthy controls and women with PCOS.
Fig 2.
Representative MS/MS spectra of peptides from upregulated protein SERPINA1.
Inset shows relative intensities of reporter ions.
Fig 3.
Representative MS/MS spectra of peptides from downregulated protein ITIH4.
Inset shows relative intensities of reporter ions.
Table 3.
List of differential proteins obtained from the follicular fluid glycoproteins enriched using a lectin-based approach and subsequent LC-nano-ESI-MS/MS.
Fig 4.
Validation of SERPINA1 upregulation (A) and ITIH4 downregulation (B), at protein level in follicular fluid by immunoblotting.
Each lane was loaded with 10 μg follicular fluid protein obtained from women with PCOS and controls. Lanes designated as C are control samples and as P are PCOS follicular fluid. The total protein load was used as loading control. Data are represented as mean ± S.D. Statistical comparison was done using Mann Whitney U tests. P values <0.05 are considered statistically significant.
Fig 5.
Relative gene expressions of ITIH4 and SERPINA1 in the GCs of women with PCOS and control.
Data are represented as mean ± S.D. Statistical comparison was done using Mann Whitney U tests. P values <0.05 are considered statistically significant.