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Fig 1.

16S rRNA based phylogeny analysis of 32 Mycobacteria strains belonging to different species: Differentiation into rapid and lowly growing Mycobacteria.

The 16S rRNA gene-based phylogenetic tree clearly distinguishes Mycobacterium grouping based on the growth rates. UM_RHS and UM_ NYF were clustered in the rapid growing mycobacterial group.

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Fig 1 Expand

Fig 2.

Supermatrix tree of five markers (hsp65, rpoB, tuf, sodA and 16S rRNA).

UM_RHS and UM_NYF are closest to M. cosmeticum, supported by a high bootstrap value of 85%.

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Fig 2 Expand

Fig 3.

Average nucleotide analysis for 37 Mycobacterium species.

The ANI values of UM_NYF and UM_RHS against M. cosmeticum DSM44829 are above 95%, supporting that these strains belong to the species M. cosmeticum.

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Fig 3 Expand

Fig 4.

RAST functional categories of UM_RHS and UM_NYF genes.

Number of genes in UM_RHS in comparison to UM_NYF which belong to specific RAST functional categories with M. cosmeticum DSM 44829 as the reference genome.

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Fig 4 Expand

Table 1.

RNAs identified by RAST in the genomes of UM_NYF and UM_RHS.

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Table 1 Expand

Table 2.

RAST predicted genes related to gene transfer in the genomes of UM_NYF and UM_RHS.

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Table 2 Expand

Fig 5.

Genomic structure of M. cosmeticum genomes.

The genome structures are generally conserved among three studied genomes (UM_RHS, UM_NYF and DSM44829).

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Fig 5 Expand

Fig 6.

Gene family distribution.

The M. cosmeticum DSM 44829, UM_RHS and UM_NYF have generally shared a high number of common gene families.

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Fig 6 Expand

Fig 7.

Predicted virulence genes in the genomes of M. cosmeticum DSM44829, UM_RHS and UM_NYF.

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Fig 7 Expand