Fig 1.
Chemical structures of dacomitinib and lapatinib (IS).
Fig 2.
MRM mass spectrum transitions of DMB (A) and lapatinib (IS) (B).
Fig 3.
Overlaid MRM chromatograms of fifteen calibration standards of DMB (2–500 ng/mL) and IS (100 ng/mL).
Fig 4.
MRM chromatograms of blank (A), blank + IS (B), and DMB 15 ng/mL LQC (C).
The blank RLM matrix revealed no matrix interference.
Fig 5.
DMB LLQC MRM chromatogram revealing high S/N.
Table 1.
Data of back-calculated DMB concentrations for the calibration standards from the RLM matrix.
Table 2.
Precision and accuracy (intra-day and inter-day) of the developed assays.
Table 3.
RLMs matrix effect on the DMB analysis.
Table 4.
Stability of dacomitinib (DMB) under different storage conditions.
Fig 6.
Metabolic stability curve of DMB treated with RLMs.
Table 5.
Metabolic stability parameters for DMB incubation with RLMs.