Fig 1.
Isolated salivary gland of Coreus marginatus (ex vivo, reflected light).
ag–accessory Salivary gland; al–anterior lobe of the principal Salivary gland; ll–lateral lobe of the principal Salivary gland; ml–median lobe of the principal Salivary gland; pl–posterior lobe of the principal Salivary gland; sd–principal duct of the Salivary gland. Scale bar 3 mm.
Fig 2.
Maximum likelihood phylogenetic trees reconstructed using different molecular markers.
A, SSU rRNA; B, gGAPDH; C, HSP83. Numbers at nodes indicate posterior probability and bootstrap percentage, respectively. Values less than 0.5 and 50% are replaced with dashes. Nodes having 1.0 posterior probability, 100% bootstrap support are marked with black circles. Double-crossed branches are at 50% of their original lengths. The trees are rooted with the sequences of Herpetomonas and Lafontella spp. (shown in grey). The scale bar represents number of substitutions per site. Phytomonas lipae is highlighted and P. oxycareni is boxed. The known/supposed parasitized plant host organs and tissues are indicated in C.
Fig 3.
Morphology of Phytomonas lipae (light microscopy, SEM and TEM).
A—C. Phytomonas lipae in the salivary gland of C. marginatus; D. Promastigote of P. lipae from the M1 segment of the host's midgut; E—H. P. lipae in the axenic culture Cor4. A–ex vivo, DIC; B, D, E–Giemsa; C–overlaid DIC and DAPI; F, G–SEM, H–TEM. en–endomastigote; fl–flagellum; fp–flagellar pocket; gl–glycosomes; kp–kinetoplast; nu–nucleus; pm–promastigote. Scale bars 20 μm (A); 10 μm (B—E); 5 μm (F); 2 μm (G, H).
Table 1.
Morphometry of different cell types of P. lipae from culture, different populations and different organs of the hosts (N = 25).
Fig 4.
Phytomonas lipae in the salivary glands of C. marginatus (TEM).
A.–Promastigotes of P. lipae in the coelomic epithelium; B. P. lipae in the cytoplasm of a salivary gland's cell; C–Promastigotes in the lumen. bl–basal lamina; ch–host's salivary gland cell; ep–coelomic epithelium; he–hemocoel; lu–lumen of the salivary gland; mc–muscle cells; mv–microvilli; tr–trachea. Other abbreviations are as in Fig 3. Scale bars: 2 μm (A, C); 2.5 μm (B).