Table 1.
Summary of sequencing outcomes from grain enlargement stage.
Fig 1.
Hierarchical clustering analysis of DEGs between T05 and T06.
T05, 5 DAP; T06, 14 DAP. A total of 9,358 genes were identified at two developmental stages. The color key represents the FPKM (Fragments Per Kilobase of exon per Million fragments mapped) normalized log2 transformed counts. Red and blue represent high and low expression. Each row represents a gene.
Fig 2.
Number of DEGs located at different chromosome on subgenome A, B and D.
Fig 3.
Histogram of Gene Ontology (GO) classifications of DEGs between 5 DAP and 14 DAP.
A total of 7,349 DEGs were assigned to three main GO functional categories and then divided into 54 sub-categories.
Fig 4.
Clusters of orthologous groups (COG) function classification of DEGs between 5 DAP and 14 DAP.
A total of 2,968 DEGs were distributed across 25 COG categories.
Fig 5.
Clusters of KEGG function classification of DEGs.
A total of 1,920 DEGs were mapped to 107 KEGG pathways.
Table 2.
Functional annotation of new genes and differentially expressed genes in the COG, GO, KEGG, Swiss-Prot and NR databases.
Fig 6.
KEGG pathway analysis identified the ubiquitin-mediated proteolysis pathway (ko04120) and proteasome pathway (ko03050) for DEGs between 5 DAP and 14DAP.
Red, green and blue indicate genes expression increase, decrease and mixed change at 14 DAP, respectively. (A) ko04120, and (B) ko03050.
Table 3.
Summary of DEGs related to grain size/weight (ubiquitin-mediated proteolysis pathway and proteasome pathway) in the KEGG database between T05 and T06.
Fig 7.
qRT-PCR analysis of 8 random selectively mRNA expression from transcriptome database.
Values are the mean ± SD (standard deviation) of three independent experiments. T05, 5 DAP; T06, 14 DAP.