Fig 1.
Fabrication scheme of Se-NP decorated TiO2 nanotube film.
TiO2 nanotubes were fabricated via anodic oxidation of 500 nm thick titanium layer deposited on silicon wafer. Nanotubes were decorated with different concentrations of selenium nanoparticles.
Fig 2.
SEM images of fabricated nanostructures.
Annealed TiO2 nanotubes (A), their cross section (B), and selenium nanoparticles (C). TiO2 nanotubes decorated with Se-Low (D), Se-Medium (E) and Se-High (F) concentrations of nanoparticles.
Fig 3.
Boxplot of normal size distributions of nanoparticle (A) and nanotube (B). Both the boxplot and Shapiro-Wilk test of normality suggested normal distributions.
Table 1.
XPS analysis of selenium decorated TiO2 nanotubes.
Relative percentage of selected elements calculated from narrow spectra.
Fig 4.
The cumulative release profile of selenium.
Individual samples were measured with ICP-MS technique. Measured release was highly dependent on initial SeNPs concentrations.
Fig 5.
Bacterial assay of viability expressed in colony forming units.
Se nanoparticle decorated TiO2 -TNTs were exposed to gram negative bacteria E. coli for 4 hours and the antibacterial effect Se-TNTs was compared with undecorated TNTs. * indicates significant difference between compared samples (p = 0.05).
Fig 6.
Adhesion and morphology of MG-63 (cancer) and NIH/3T3 (normal) cells cultured on Se-NPs decorated TiO2 nanotubes.
DIC images were taken after 3 and 24 hours cultivation. Live/dead staining of cells was performed with calcein (green color) and propidium iodide (red color) at the day 6.
Fig 7.
XTT viability assay of MG-63 (B) and NIH/3T3 (A) cells cultured on Se-NPs decorated TiO2 nanotubes. * indicates significant difference between compared samples (p = 0.05).