Table 1.
Setup specifications.
Fig 1.
a) Connections between the custom PCB board and the INTAN RHD200-EVAL board, showing the electroporation, the acquisition and the computer transfer paths. b) Sketches in the center show all the components of the custom PCB board and the connections to external devices. c) Photographs depict the complete setup.
Fig 2.
Impedance of gold electrodes from commercial MEA chips.
The impedances were measured with the setup at fixed frequencies of 100, 1000 and 7500 Hz. The mean values of the impedance measured at each frequency are shown as blue dots connected by a discontinuous line. The values of the standard deviations are indicated by the error bars.
Fig 3.
Sample traces acquired from a commercial MEA chip.
Each panel shows a 3-second long voltage trace acquired from a different channel.
Fig 4.
a) Waveforms of detected spikes with different amplitudes. b) 1. Distribution of the average spike amplitudes per electrode. 2. Distribution of spike frequencies per electrode. c) Propagation of sequential APs across the surface of an MEA chip. The detected spikes were placed on the 2D location of the electrodes where they were detected. In the image, four spikes detected sequentially in time are shown in their location on the chip surface. The direction of the arrows indicates the temporal order of the spikes, with the time between APs annotated next to the arrows.
Fig 5.
a) Typical intracellular waveform observed immediately after applying the electroporation protocol. b) Average spikes detected before and at different times after electroporation. Each panel shows the average of all the spikes detected in the 10 seconds after the indicated time. The average waveform is shown in blue whereas the mean plus or minus the standard deviation on each point is shown in red to indicate the magnitude of the uncertainty. The number of spikes used to obtain the average is indicated on each panel.