Fig 1.
2DE gel protein expression profiles of human hair shaft protein.
(A) Conventional protocol. Proteins were separated in the first dimension on an IPG strip (pH 4–7; 13 cm) and in the second dimension on a hand-cast 8–15% gradient gel. Gel was silver-stained and scanned using Image Scanner III. (B) Modified protocol. Proteins were separated in the first dimension on an IPG strip (pH 3–10; 7 cm) and in the second dimension on a pre-cast 4–12% gradient gel. Gel was Sypro Ruby-stained and scanned using Typhoon FLA 9000 Scanner. Both gels were loaded with 100 μg of hair protein with reference to result obtained from protein quantification.
Table 1.
Number of protein spots detected in both conventional and modified protocol.
Table 2.
Overview of the different experimental procedures used in both conventional and modified protocol.