Fig 1.
Brucella AMOS-PCR profiles: In order to identify Brucella species and biovars, for each strain, 4 PCR have been performed: AMOS multiplex PCR, Omp 2a PCR, Omp 2b PCR and Omp 31 PCR.
In this image, this four PCR have been grouped to obtain a good glance and easily identify the Brucella profile. Lane 1 and 22 Marker molecular weights; Lane 2–5: Amos (2)-Omp2a (3)-Omp2b (4)-Omp31 (5) B. abortus biovar 3,5,6,9; Lane 6–9: Amos (6)-Omp2a (7)-Omp2b (8)-Omp31 (9) B. suis biovar 2,3,4,5; Lane 10–13: Amos (10)-Omp2a (11)-Omp2b (12)-Omp31 (13) B. melitensis biovar 1,2,3; Lane 14–17: Amos (14)-Omp2a (15)-Omp2b (16)-Omp31 (17) B. abortus biovar 1,2,4; Lane 18–21: Amos (18)-Omp2a (19)-Omp2b (20)-Omp31 (21) B. ovis.
Table 1.
Total numbers of B. abortus, B. melitensis, B. ovis, B. suis, and B. ceti biovars isolated from 2007 to 2015.
Table 2.
Percentages of B. abortus, B. melitensis, B. ovis, B. suis, and B. ceti biovars isolated from 2007 to 2015.
Fig 2.
Geographical distribution of the 2,981 Brucella strains isolated from cattle in Italy from 2007 to 2015.
Fig 3.
Geographical distribution of the 322 Brucella strains isolated from water buffalo in Italy from 2007 to 2015.
Fig 4.
Geographical distribution of the 2,342 Brucella strains isolated from sheep and goats in Italy from 2007 to 2015.
Fig 5.
Geographical distribution of the 173 Brucella strains isolated from wild boars in Italy from 2007 to 2015.
Fig 6.
Geographical distribution of the 33 Brucella strains isolated from humans, pigs, dolphins, and horses in Italy from 2007 to 2015.