Fig 1.
(a) Molecular structures of the hop compounds xanthohumol (XN) and xanthohumol C (XNC) obtained from the xanthohumol-enriched hop extract Xantho-Flav (XF). (b) Overview of the total workflow of the study.
Fig 2.
Relative cell growth of MCF-7 cells determined by MTS-assay after 2 (a), 4 (b) and 6 (c) days of incubation at different concentrations of XF, XN and XNC (*statistical significance to control, p-value<0.05).
Fig 3.
Cytotoxic effect on MCF-7 cells after 2 (a), 4 (b) and 6 (c) days of incubation with different concentrations of XF, XN and XNC (* statistical significance to control, p-value<0.05).
Note: Experiments with XN treatment in Fig 3B were only performed in duplicate.
Fig 4.
Global proteome characterization from MCF-7 cells after treatment with XN and XNC at IC50 concentration.
(a) Comparison of the number of identified, quantified, and differentially expressed (DE) proteins. (b) Comparison of shared up- and downregulated DE proteins (c) Predicted cellular localization of quantified and DE proteins in percentage based on iBAQ intensities (d) Predicted molecular function of quantified and DE proteins in percentage based on iBAQ intensities (e) Predicted biological process of quantified and DE proteins in percentage based on iBAQ intensities. (*significant difference, p < 0.005; **highly significant difference, p < 0.001).
Fig 5.
Volcano plots represent significant differences between MXF-7 treated cells (group 1) and DMSO control cells (group 2) for XNC (a) and XN (b).
Significant up- and downregulated proteins that could be assigned to one function or family are marked. The protein names are listed in supporting information S6–S9 Files.