Fig 1.
Localizing the boundaries of the head block.
Based on the CT-scans, three-dimensional reconstructions were made to determine the borders of the head block (red rectangle). Lateral (A) and dorsal (B) views.
Fig 2.
Subtracting the vessels from a cryosectioned image: Effects of the applied filters on the same slice.
Original color image (A), and after selective filtering for the arteries (B) and veins (C).
Fig 3.
Transverse section at the level of the orbit (A). Blue frames on image (A) show the zoomed regions (B and C). Close-up views show the detailedness of the ethmoturbinates in the nasal cavity (B) and the intrinsic muscles of the tongue (C).
Fig 4.
Transverse section at the mid-thalamic level (A). Blue frame on image (A) shows the zoomed region (B). Close-up view (B) shows the detailedness of the vessels (like sinus cavernosus, internal carotid arteries and branches of circulus arteriosus). https://doi.org/10.6084/m9.figshare.c.4365566.v1.
Fig 5.
Transverse section of a dog brain showing discoloration and frozen line artefacts.
Fig 6.
Fiber-formation: Demonstrating the importance of proper cooling.
Arrows show the fibrous-tendinous tissue of the temporal muscle attaching on the coronoid process of the mandible (A). After the treatment with dry ice and liquid nitrogen these fibers harden enough to eliminate them during the upcoming sectionings (B).
Fig 7.
Transverse section at the level of the rostral commissure (A) and at the level of lateral geniculate body (B).
(1) Cingulate gyrus. (2) Corpus callosum. (3) Caudate nucleus. (4) Claustrum. (5) Putamen. (6) Globus pallidus. (7) Septal area. (8) Rostral commissure. (9) Optic tract. (10) Hippocampus. (11) Lateral geniculate nucleus.
Fig 8.
Reconstruction based on the original transverse cryosectioned images.
(A) Transverse plane (original image). (B) Sagittal plane (reconstructed image). (C) Dorsal plane (reconstructed image). (D) Three-dimensional composite picture of the orthogonal views.
Fig 9.
The three co-registered imaging modalities in a perspective view.
MR is in the transverse plane (yellow frame), CT is in the sagittal plane (blue frame), cryosectioned image is in the dorsal plane (orange frame).
Fig 10.
Comparing the result of different imaging modalities at the same level.
(A) At the level of the olfactory peduncle. (B) At the level of the hypophysis. (C) At the level of the cerebellum. (1) Cryosectioned image. (2) CT with brain window. (3) T2-weighted MRI. (4) CT with bone window.
Fig 11.
Arteries (in red) and veins (in blue) on the 3D-model with different detailedness.
(A) Lateral view. (B) Dorsal view. (1) Less detailed 3D-model. (2) Highly detailed 3D-model.
Fig 12.
Transverse section at the level of the frontal lobe.
Composite image with the grayscale-converted cryosectioned image and the crossing vessels from the 3D-models (arteries with red color and veins with blue color).
Fig 13.
The different imaging modalities together with the vascular 3D-models.
Composite image with the cryosectioned layer (dorsal plane, orange frame), the T2-weighted MRI (transverse plane, yellow frame), and the 3D-models of the major arteries and veins. Left rostro-lateral view.
Fig 14.
The different imaging modalities together with the vascular 3D-models.
Composite image with the cryosectioned layer (reconstructed in dorsal plane, orange frame), the T2-weighted MRI (transverse plane, yellow frame), CT-scan (sagittal plane, blue frame) and the 3D-models of the major arteries and veins. Left caudo-lateral view.
Fig 15.
Surface model of the vessels, and the extracted brain model.
Models as a whole (A), and sectioned in the transverse plane (B) and the dorsal plane (C). Arteries with red, veins with blue color.
Fig 16.
Surface model of the skull with the three main imaging modalities presented in the different orthogonal planes.
Cryosectioned image is on the dorsal (“xy”) plane. MR image is on the transverse (“xz”) plane. CT image is on the sagittal (“yz”) plane.