Fig 1.
The exposure cage apparatus was a standard mouse cage bisected with chicken wire.
Mice in the M/F and F Exposed conditions lived in the exposure cages for four weeks.
Fig 2.
Schematic of the recording apparatus.
A standard mouse cage was placed into an anechoic chamber. A condenser microphone was placed on each side of the apparatus, pointing towards the opposite wall. Mice were placed inside the recording apparatus alone for the entire duration of the recording session. Recordings lasted for five minutes following a 15 second habituation to the apparatus.
Fig 3.
Example timeline of recording sessions for one mouse.
The order of the sex of the stimulus mouse was counterbalanced across mice within each phase.
Fig 4.
Example USV spectrograms from the (a) chevron, (b) chirp, (c) complex, (d) downsweep, (e) flat, (f) inverse chevron, (g) jump, (h) multijump, and (i) upsweep categories.
Fig 5.
Number of USVs within housing conditions.
Number of USVs produced by (a) Isolated, (b) F Exposed, and (c) M/F Exposed female mice to female stimulus mice (black bars) and male stimulus mice (white bars) in phases 2 and 3. Bars represent means and error bars represent SEM.
Fig 6.
Number of USVs between housing conditions.
Number of USVs produced by Isolated mice (white bars), F Exposed mice (gray bars), and M/F Exposed mice (black bars) in all four recording sessions, averaged across phases 2 and 3. Bars represent upper and lower quartile range of number of USVs, horizontal lines represent the medians.
Fig 7.
The proportion of USVs produced by each social exposure group (Isolated = white bars, F Exposed = gray bars, and M/F Exposed = black bars) for (a) chevron, (b) chirp, (c) complex, (d) downsweep, (e) flat, (f) inverse chevron, (g) jump, (h) multijump, and (i) upsweep USV categories. Bars represent upper and lower quartile range of proportion of each USV, horizontal line represents median. * p < 0.025.
Fig 8.
Variety of USVs produced within housing conditions.
Mean number of USV types produced by (a) Isolated, (b) F Exposed, and (c) M/F Exposed mice to female stimulus mice (filled bars) and male stimulus mice (open bars) in each recording session in phases 2 and 3. Bars represent mean number of USVs emitted in a session and error bars represent SEM.
Fig 9.
Variety of USVs produced between housing conditions.
Mean number of USV types produced by Isolated (white bars), F Exposed (gray bars), and M/F Exposed mice (black bars) in all four recording sessions in phases 2 and 3. Bars represent mean number of USVs emitted in all sessions and error bars represent SEM.
Fig 10.
Features of USVs produced by mice in all three housing conditions.
(a) Duration, (b) bandwidth, and (c) peak frequency of USVs from all categories emitted by Isolated (white bars), F Exposed (gray bars), and M/F Expose (black bars) in all recording sessions. Bars represent upper and lower quartile ranges of each feature, horizontal line represents median, circle symbols represent outliers. *** p < 0.001.