Fig 1.
Routine workflow used in our laboratory.
DNA quality control is assessed for all samples. Samples that don’t reach quality criteria are assessed using Idylla assay.
Fig 2.
Decision algorithm used in our laboratory for the determination of KRAS, NRAS and BRAF mutations using the Idylla system.
Table 1.
Samples recovered by Idylla according to ΔQC.
Fig 3.
A total of 669 mCRC samples have been analyzed.
53 mCRC samples failed QC for NGS assessment and 14 samples failed a valid sequencing result and have been analyzed with Idylla KRAS cartridge. No further analysis was conducted for the 17 invalid results and the 22 samples with a KRAS mutation. 28 wild-type KRAS samples have been analyzed with Idylla NRAS-BRAF mutation test.
Table 2.
KRAS, NRAS and BRAF results using Idylla assay.
Table 3.
Samples results including QC, ΔQC, DNA concentration (ng/μL) and mutation results for KRAS, NRAS and BRAF genes using the Idylla system.