Fig 1.
Carrot variants used during the present study. A) Purple Black (PB) and B) Orange Red (OR).
Fig 2.
Flow chart of library preparation and sequencing.
Fig 3.
Total number of reads after sequencing.
Fig 4.
Total number of miRNAs in PB and OR variants.
Fig 5.
Functional annotation of predicted targets of miRNAs in OR carrot.
Graph is showing various processes under different functional categories.
Fig 6.
Functional annotation of predicted targets of miRNAs in PB carrot.
Graph is showing various processes under different functional categories.
Fig 7.
Heat map showing differential expression of miRNAs regulating a) carotenoid and b) flavonoid pathway in OR and PB.
Fig 8.
Transcript profiles of the miRNAs targeting a) carotenoid genes b) flavonoid genes and c) Transcription factor genes using qRT-PCR. For normalizing the gene expression level, tubulin was selected as an internal control. All experiments were conducted using the three technical replicates of the biological tissues. All the fold changes were calculated using ΔΔCt method that shows the change in gene expression level in the OR and PB carrot. Standard error was calculated from the average of three technical replicates of the biological tissues. Student’s t-test (P < 0.05) was performed in order to determine the significant differences in the miRNA expression levels between OR and PB sample. The significant differences (P < 0.05) obtained has been marked as * in the figure.
Fig 9.
Cleavage site detection using RLM-RACE approach.
5' RLM RACE was employed so as to map the cleavage sites. The coding sequence of the target gene was aligned with the miRNA and the arrow above the aligned sequences indicates the cleavage site.