Fig 1.
Clinical and histological phenotype of Greyhounds with BTS.
(a) Affected dog with alopecia on the caudal and lateral thighs extending below the lateral hock area; (b) Affected dog with alopecia on the caudal and lateral thighs, the lateral hind leg down to the hock, the ventral abdomen and the ventral chest; (c) Normal telogen HF; (d) Telogen HF with an increased amount of trichilemmal keratin at the proximal end of the follicle. The more distal part of the HS is not anchored by trichilemmal keratin; (e) Horizontally broken telogen HF, which is not anchored by trichilemmal keratin and abundant keratin at the base of the follicle.
Fig 2.
Boxplots illustrating the percentage of fractured hair shafts plucked from the thigh and back of affected and control dogs.
The box includes the median of the data and the box edges are the 25th and 75th percentiles. The vertical size of the boxes displays the interquartile range (IQR); the whiskers represent the minimum and maximum values that do not exceed 1.5 x IQR from the median. ** p < 0.01 *** p < 0.001.
Fig 3.
Structural abnormalities in hair fibers of Greyhounds identified by scanning electron microscopy.
(a) Intact telogen hair root of a control Greyhound, (b-d) abnormal HSs from Greyhounds with BTS (b) Clean transverse fracture (trichoschisis); (c) Central longitudinal splitting (central trichoptilosis). (d) Irregular surface of the cuticle; (e) Longitudinal grooves.
Fig 4.
Canine hair proteins identified by nLC-MS/MS.
(a) Hierarchical clustering of LFQ protein intensities. Highly abundant proteins are shown in red, low abundances in green, intermediate values in different shades of red and green and missing protein values are displayed in white. BTS affected dogs and control dogs form two distinct clusters. (b) Volcano plot representing the results of the T-test performed on LFQ intensities of control vs BTS. Differentially expressed proteins with a FDR < 0.05 and a log2 fold change cut-off value of 1 are labeled.
Table 1.
Differential gene and protein expression in hair shafts and skin of dogs with BTS vs controls.