Fig 1.
FACS analysis of cell progenitor populations over time.
(A) Schematic of the classical HSC differentiation hierarchy where early progenitors (identified as LSK) differentiate into CMPs before maturing into fully developed myeloid cells (Terminal). (C-D) Total number of each cell sub-population (LSK, CMP, Terminal) in culture over time. While the LSK cells recover some of their numbers, CMP population show variable kinetics but overall low numbers, and Terminal cells grow at an exponential rate. Error bars represent standard error of mean.
Fig 2.
Computational set up of a 3-state cell model and experimental verification.
(A) A 3-state computational model featuring the LSK, CMP and Terminal hematopoietic cells. Culture dynamics is influenced via feedback from cell-secreted biomolecules that stimulate and/or inhibit the proliferation and differentiation rates of each cell type. Additionally, a fraction of LSKs would be available to transition directly into Terminal (‘jump’). (B-D) The model simulation is able to capture the dynamics and scale of the experimental data for all 3 cell profiles (B: LSK; C: CMP; D: Terminal). The initial model further predicted a peak in CMP population prior to day 1 of culture. (E-G) Further experiments were performed to capture data before and after the 1 day data point (12hr; 48hr) for all three cell sub-populations (E: LSK; F: CMP; G: Terminal) to substantiate the initial model predictions. Error bars represent standard error of mean.
Fig 3.
Model fit and predictions for intermediate media exchange frequencies.
(A—C) Undisturbed culture conditions over the 9 day period (denoted as 10 day), led to a drop in all cell populations as compared to the 2-day media exchange. Model parameters were optimized to obtain a high degree of correlation with experimental data simply by changing media frequency change in the model to 10 days. The model was able to predict the system response to a 5-day media exchange for all the 3 cell populations, without any parameter optimization. Subtle differences in cell numbers were captured over long term culture. Inset: a close up of the model and data fit between days 7–9. Error bars represent standard deviation.
Fig 4.
A five state model framework for HSC lineage specification.
(A) A schematic of the classical HSC differentiation hierarchy where long-term progenitors (LT-HSC) differentiate into short-term (ST-HSC) progenitors, then multipotent (MPP), then common myeloid progenitors (CMP) before maturing into fully developed myeloid cells (Terminal). (B) A 5-state computational model featuring the LT-HSC, ST-HSC, MPP, CMP, and Terminal cell types. Culture dynamics may be influenced via feedback from cell-secreted biomolecules that stimulate and/or inhibit the proliferation and differentiation rates of each cell type. Additionally, a fraction of ST-HSCs and MPPs would be available to transition directly into Terminal cells without going through CMP differentiation (‘jump’). (C-G) The model simulation is able to capture the dynamics and scale of the experimental data for all 5 cell profiles (C: LT-HSC; D: ST-HSC; E: MPP; F: CMP; G: Terminal) over a 9 day period. Error bars represent standard deviation.
Fig 5.
Parameter sensitivity matrix of the 5-state HSC differentiation model, broken down by cell state (LT-HSC, ST-HSC, MPP, CMP, Terminal) and type of model parameter.
Red nodes in the matrix indicate that model sensitivity is >1% for a 1% change in parameter values. This is indicative of high parameter sensitivity and system instability, but also design parameters for future experimental optimization. Terminal cells, on account of their large and heterogeneous populations, are relatively insensitive to several model parameters except their proliferation rates (PRTermmax).