Fig 1.
Experimental overview of gamma radiation exposures.
Embryos were exposed to gamma radiation (nominal dose rate 10 mGy/h) during blastula to early gastrula stage (5.5 hpf for zebrafish and 60 degree days for Atlantic salmon). Adult zebrafish were exposed for 27 days to affect gametogenesis prior to preduction of F1 and F2 generations. Histone PTM enrichment was measured with chromatin immunoprecipitation and real time PCR changes relative to control is indicated by a green or orange color.
Fig 2.
ChIP of directly exposed zebrafish embryos.
Fold change enrichment of indicated histone PTMs in directly exposed zebrafish embryos (10.9 mGy/h for 3h) relative to unexposed embryos (5.5 hpf stage). Nucleotide positions are indicated relative to TSS. *p<0.05. Error bars reflect SEM.
Fig 3.
ChIP of ovaries from exposed parental generation.
Enrichment of H3 and H3K4me3 in zebrafish ovaries after 27 day exposure during gametogenesis (control, 8.7 and 53 mGy/h). Enrichment is measured upstream of TSS, at TSS and downstream of TSS (coordinates given in Fig 1). *p<0.05. Error bars reflect SEM.
Fig 4.
ChIP analysis of F1 and F2 offspring of exposed parents.
Fold change enrichment (relative to controls) of indicated histone PTMs in F1 and F2 zebrafish embryos (5.5 hpf) after parental gamma radiation exposure. Nucleotide positions indicated relative to TSS. *p<0.05. Error bars reflect SEM.
Fig 5.
Atlantic salmon embryo (60 degree days; 50% epiboly) ChIP analysis enrichment at TSS of IgG and H3K4me3 after exposures for 10 days at 0–30 mGy/h. Error bars reflect the SEM of two technical replicates on one biological sample made from pools of 60 embryos.